TY - JOUR
T1 - Menaquinone-4 accumulation in various tissues after an oral administration of phylloquinone in Wistar rats
AU - Yamamoto, Ryuta
AU - Komai, Michio
AU - Kojima, Kouki
AU - Furukawa, Yuji
AU - Kimura, Shuichi
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997
Y1 - 1997
N2 - The distributions of phylloquinone (PK) and menaquinone-4 (MK-4) in various tissues were assessed after the oral administration of phylloquinone. Wistar rats were fed a vitamin-K-deficient diet for nine days, fasted for 24 h and then given phylloquinone orally at 4 mg/kg body weight. Rats were sacrificed 0, 6, 12 and 24 h after the administration, and an analysis was made of the vitamin K analogues in the plasma, liver, brain, testis, kidney and spleen. The phylloquinone concentration in plasma and the tissues reached a peak 6 h after the oral administration of phylloquinone. By contrast, the concentration of MK-4 peaked in the liver, plasma and spleen at 12 h, and in brain and testis at 24 h. This data suggests that the ingested phylloquinone was probably converted into MK-4 within the tissues themselves, rather than via hepatic metabolism. The evidence for this is that, after phylloquinone administration, (i) in each of the tissues, the MK-4 concentration increased much more slowly than that of phylloquinone, and (ii) the MK-4 concentration in the plasma and liver reached only much lower levels than those seen in other tissues.
AB - The distributions of phylloquinone (PK) and menaquinone-4 (MK-4) in various tissues were assessed after the oral administration of phylloquinone. Wistar rats were fed a vitamin-K-deficient diet for nine days, fasted for 24 h and then given phylloquinone orally at 4 mg/kg body weight. Rats were sacrificed 0, 6, 12 and 24 h after the administration, and an analysis was made of the vitamin K analogues in the plasma, liver, brain, testis, kidney and spleen. The phylloquinone concentration in plasma and the tissues reached a peak 6 h after the oral administration of phylloquinone. By contrast, the concentration of MK-4 peaked in the liver, plasma and spleen at 12 h, and in brain and testis at 24 h. This data suggests that the ingested phylloquinone was probably converted into MK-4 within the tissues themselves, rather than via hepatic metabolism. The evidence for this is that, after phylloquinone administration, (i) in each of the tissues, the MK-4 concentration increased much more slowly than that of phylloquinone, and (ii) the MK-4 concentration in the plasma and liver reached only much lower levels than those seen in other tissues.
KW - brain
KW - kidney
KW - liver
KW - menaquinone-4 (MK-4)
KW - metabolic conversion
KW - phylloquinone (PK)
KW - rats
KW - spleen
KW - testis
KW - vitamin K
UR - http://www.scopus.com/inward/record.url?scp=0030932090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030932090&partnerID=8YFLogxK
U2 - 10.3177/jnsv.43.133
DO - 10.3177/jnsv.43.133
M3 - Article
C2 - 9151247
AN - SCOPUS:0030932090
VL - 43
SP - 133
EP - 143
JO - The Journal of vitaminology
JF - The Journal of vitaminology
SN - 0301-4800
IS - 1
ER -