Menaquinone-4 accumulation in various tissues after an oral administration of phylloquinone in Wistar rats

Ryuta Yamamoto, Michio Komai, Kouki Kojima, Yuji Furukawa, Shuichi Kimura

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    The distributions of phylloquinone (PK) and menaquinone-4 (MK-4) in various tissues were assessed after the oral administration of phylloquinone. Wistar rats were fed a vitamin-K-deficient diet for nine days, fasted for 24 h and then given phylloquinone orally at 4 mg/kg body weight. Rats were sacrificed 0, 6, 12 and 24 h after the administration, and an analysis was made of the vitamin K analogues in the plasma, liver, brain, testis, kidney and spleen. The phylloquinone concentration in plasma and the tissues reached a peak 6 h after the oral administration of phylloquinone. By contrast, the concentration of MK-4 peaked in the liver, plasma and spleen at 12 h, and in brain and testis at 24 h. This data suggests that the ingested phylloquinone was probably converted into MK-4 within the tissues themselves, rather than via hepatic metabolism. The evidence for this is that, after phylloquinone administration, (i) in each of the tissues, the MK-4 concentration increased much more slowly than that of phylloquinone, and (ii) the MK-4 concentration in the plasma and liver reached only much lower levels than those seen in other tissues.

    Original languageEnglish
    Pages (from-to)133-143
    Number of pages11
    JournalJournal of nutritional science and vitaminology
    Volume43
    Issue number1
    DOIs
    Publication statusPublished - 1997

    Keywords

    • brain
    • kidney
    • liver
    • menaquinone-4 (MK-4)
    • metabolic conversion
    • phylloquinone (PK)
    • rats
    • spleen
    • testis
    • vitamin K

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Nutrition and Dietetics

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