TY - JOUR
T1 - Membrane topology of the electrogenic aspartate-alanine antiporter AspT of Tetragenococcus halophilus
AU - Nanatani, Kei
AU - Ohonishi, Fumito
AU - Yoneyama, Hiroshi
AU - Nakajima, Tasuku
AU - Abe, Keietsu
PY - 2005/3/4
Y1 - 2005/3/4
N2 - AspT is an electrogenic aspartate:alanine exchange protein that represents the vectorial component of a proton-motive metabolic cycle found in some strains of Tetragenococcus halophilus. AspT is the sole member of a new family, the Aspartate: Alanine Exchanger (AAE) family, in secondary transporters, according to the computational classification proposed by Saier et al. (http://www.biology.ucsd.edu/~msaier/transport/). We analyzed the topology of AspT biochemically, by using fusion methods in combination with alkaline phosphatase or β-lactamase. These results suggested that AspT has a unique topology; 8 TMS, a large cytoplasmic loop (183 amino acids) between TMS5 and TMS6, and N- and C-termini that both face the periplasm. These results demonstrated a unique 2D-structure of AspT as the novel AAE family.
AB - AspT is an electrogenic aspartate:alanine exchange protein that represents the vectorial component of a proton-motive metabolic cycle found in some strains of Tetragenococcus halophilus. AspT is the sole member of a new family, the Aspartate: Alanine Exchanger (AAE) family, in secondary transporters, according to the computational classification proposed by Saier et al. (http://www.biology.ucsd.edu/~msaier/transport/). We analyzed the topology of AspT biochemically, by using fusion methods in combination with alkaline phosphatase or β-lactamase. These results suggested that AspT has a unique topology; 8 TMS, a large cytoplasmic loop (183 amino acids) between TMS5 and TMS6, and N- and C-termini that both face the periplasm. These results demonstrated a unique 2D-structure of AspT as the novel AAE family.
KW - Aspartate:Alanine Antiporter
KW - Electrogenic
KW - Fusion methods
KW - Membrane topology
KW - Proton-motive force
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U2 - 10.1016/j.bbrc.2004.12.133
DO - 10.1016/j.bbrc.2004.12.133
M3 - Article
C2 - 15670744
AN - SCOPUS:12844256463
VL - 328
SP - 20
EP - 26
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -