Membrane surface-enhanced Raman spectroscopy for sensitive detection of molecular behavior of lipid assemblies

Keishi Suga, Tomohiro Yoshida, Haruyuki Ishii, Yukihiro Okamoto, Daisuke Nagao, Mikio Konno, Hiroshi Umakoshi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The dynamic properties of phospholipid (PL) membranes (phase state and phase transition) play crucial roles in biological systems. However, highly sensitive, direct analytical methods that shed light on the nature of lipids and their assemblies have not been developed to date. Here, we describe the analysis of PL-modified Au nanoparticles (Au@PL) using membrane surface-enhanced Raman spectroscopy (MSERS) and report the properties of the self-assembled PL membranes on the Au nanoparticle. The Raman intensity per PL concentration increased by 50-170 times with Au@PL, as compared to large unilamellar vesicles (LUVs) at the same PL concentration. The phase state and phase transition temperature of the PL membrane of Au@PL were investigated by analyzing the Raman peak ratio (R = I2882/I2930). The enhancement at 714 cm-1 (EF(714)) varied with the hydrocarbon chain length of the PLs and the assembled degree of Au@PLs. In calculation, the EF(714),assembled was estimated to be 111-142 when the distance between AuNPs was 7.0-7.5 nm, which was correlated to the speculative enhancement factor, suggesting that the assembly of the Au@PLs contributed to the MSERS.

Original languageEnglish
Pages (from-to)4772-4780
Number of pages9
JournalAnalytical Chemistry
Volume87
Issue number9
DOIs
Publication statusPublished - 2015 May 5

ASJC Scopus subject areas

  • Analytical Chemistry

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