Membrane potential microscope for functional brain imaging

Ichiro Takashima, Riichi Kajiwara, Toshio Iijima

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We have developed a membrane potential microscope for monitoring neural activity in the cortex stained with a voltage-sensitive dye. The microscope captures images using a high-speed camera, and its optical apparatus is designed for experiments on small primates (monkeys). The camera uses a custom-made CCD chip that has 1776H × 1626V pixels and 16 parallel output terminals. By incorporating the pixel-binning technique, the camera produced 296H × 271V pixel-images at a rate of approximately 2.5 ms/frame. The acquired images were then processed to produce functional brain images of somewhat reduced resolutions (the resolution reduction being as a consequence of spatio-temporal filtering). Special lenses for the optical apparatus gave a nearly uniform illumination over the wide CCD active area of 20.4 × 18.7 mm. Tests of the microscope showed that it could visualize neural activity in the somatosensory cortex of macaque monkeys (Macaca fuscata).

    Original languageEnglish
    Pages (from-to)476-481
    Number of pages6
    JournalKyokai Joho Imeji Zasshi/Journal of the Institute of Image Information and Television Engineers
    Volume56
    Issue number3
    DOIs
    Publication statusPublished - 2002 Mar

    ASJC Scopus subject areas

    • Media Technology
    • Computer Science Applications
    • Electrical and Electronic Engineering

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