TY - JOUR
T1 - Members of the ErbB receptor tyrosine kinases are involved in germ cell development in fetal mouse gonads
AU - Toyoda-Ohno, Hiroko
AU - Obinata, Masuo
AU - Matsui, Yasuhisa
N1 - Funding Information:
We thank Dr. Y. Nishimune for the TRA98 monoclonal antibody, Dr. N. Nakayama for hNDFβ, and Dr. F. Cuzin for the 15P1 cell. This work was supported in part by grants-in-aid from the Ministry of Education, Science, Sports, and Culture of Japan and by the Science and Technology Agency of Japan, with Special Coordinating Funds for Promoting Science and Technology.
PY - 1999/11/15
Y1 - 1999/11/15
N2 - To isolate the genes involved in mouse primordial germ cell (PGC) development, we carried out subtraction cDNA cloning between PGC-derived embryonic germ (EG) cells and inner cell mass-derived embryonic stem cells. Among the genes preferentially expressed in EG cells, we found a gene encoding a receptor tyrosine kinase ErbB3. By in situ hybridization and immunohistochemical staining, the expression of ErbB3 as well as that of ErbB2, a coreceptor for ErbB3, was detected in PGCs in genital ridges at 12.5 dpc (days postcoitum). The expression was, however, downregulated at 14.5 dpc when the PGCs underwent growth cessation. Neuregulin-β, a ligand for ErbB2 and ErbB3, was also expressed in genital ridges. In addition, a recombinant Neuregulin-β enhanced the number of PGCs in 12.5-dpc embryos in culture. Taken together, these observations suggest that ErbB signaling controls the growth or survival of PGCs in genital ridges.
AB - To isolate the genes involved in mouse primordial germ cell (PGC) development, we carried out subtraction cDNA cloning between PGC-derived embryonic germ (EG) cells and inner cell mass-derived embryonic stem cells. Among the genes preferentially expressed in EG cells, we found a gene encoding a receptor tyrosine kinase ErbB3. By in situ hybridization and immunohistochemical staining, the expression of ErbB3 as well as that of ErbB2, a coreceptor for ErbB3, was detected in PGCs in genital ridges at 12.5 dpc (days postcoitum). The expression was, however, downregulated at 14.5 dpc when the PGCs underwent growth cessation. Neuregulin-β, a ligand for ErbB2 and ErbB3, was also expressed in genital ridges. In addition, a recombinant Neuregulin-β enhanced the number of PGCs in 12.5-dpc embryos in culture. Taken together, these observations suggest that ErbB signaling controls the growth or survival of PGCs in genital ridges.
KW - EG cell
KW - ES cell
KW - ErbB3
KW - Neuregulin
KW - Primordial germ cell
KW - Subtraction cDNA cloning
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U2 - 10.1006/dbio.1999.9482
DO - 10.1006/dbio.1999.9482
M3 - Article
C2 - 10545246
AN - SCOPUS:0033571394
VL - 215
SP - 399
EP - 406
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -