Members of the ErbB receptor tyrosine kinases are involved in germ cell development in fetal mouse gonads

Hiroko Toyoda-Ohno, Masuo Obinata, Yasuhisa Matsui

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

To isolate the genes involved in mouse primordial germ cell (PGC) development, we carried out subtraction cDNA cloning between PGC-derived embryonic germ (EG) cells and inner cell mass-derived embryonic stem cells. Among the genes preferentially expressed in EG cells, we found a gene encoding a receptor tyrosine kinase ErbB3. By in situ hybridization and immunohistochemical staining, the expression of ErbB3 as well as that of ErbB2, a coreceptor for ErbB3, was detected in PGCs in genital ridges at 12.5 dpc (days postcoitum). The expression was, however, downregulated at 14.5 dpc when the PGCs underwent growth cessation. Neuregulin-β, a ligand for ErbB2 and ErbB3, was also expressed in genital ridges. In addition, a recombinant Neuregulin-β enhanced the number of PGCs in 12.5-dpc embryos in culture. Taken together, these observations suggest that ErbB signaling controls the growth or survival of PGCs in genital ridges.

Original languageEnglish
Pages (from-to)399-406
Number of pages8
JournalDevelopmental Biology
Volume215
Issue number2
DOIs
Publication statusPublished - 1999 Nov 15
Externally publishedYes

Keywords

  • EG cell
  • ES cell
  • ErbB3
  • Neuregulin
  • Primordial germ cell
  • Subtraction cDNA cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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