Melanoregulin regulates retrograde melanosome transport through interaction with the RILP-p150Glued complex in melanocytes

Norihiko Ohbayashi, Yuto Maruta, Morié Ishida, Mitsunori Fukuda

    Research output: Contribution to journalArticlepeer-review

    41 Citations (Scopus)

    Abstract

    Melanoregulin (Mreg), a product of the dilute suppressor gene, has been implicated in the regulation of melanosome transport in mammalian epidermal melanocytes, given that Mreg deficiency was found to restore peripheral melanosome distribution from perinuclear melanosome aggregation in Rab27A-deficient melanocytes. However, the function of Mreg in melanosome transport has remained unclear. Here, we show that Mreg regulates microtubule-dependent retrograde melanosome transport through the dynein- dynactin motor complex. Mreg interacted with the C-terminal domain of Rab-interacting lysosomal protein (RILP) and formed a complex with RILP and p150Glued (also known as dynactin subunit 1, DCTN1), a component of the dynein-dynactin motor complex, in cultured cells. Overexpression of Mreg, RILP or both, in normal melanocytes induced perinuclear melanosome aggregation, whereas knockdown of Mreg or functional disruption of the dynein-dynactin motor complex restored peripheral melanosome distribution in Rab27A-deficient melanocytes. These findings reveal a new mechanism by which the dynein-dynactin motor complex recognizes Mreg on mature melanosomes through interaction with RILP and is involved in the centripetal movement of melanosomes.

    Original languageEnglish
    Pages (from-to)1508-1518
    Number of pages11
    JournalJournal of cell science
    Volume125
    Issue number6
    DOIs
    Publication statusPublished - 2012 Mar 15

    Keywords

    • Dilute suppressor
    • Dynein-dynactin complex
    • Hypopigmentation
    • Microtubule-dependent transport
    • Rab27A

    ASJC Scopus subject areas

    • Cell Biology

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