Melanin-concentrating hormone in the human brain

Toraichi Mouri, Kazuhiro Takahashi, Hiroshi Kawauchi, Masahiko Sone, Kazuhito Totsune, Osamu Murakami, Keiichi Itoi, Makoto Ohneda, Hironobu Sasano, Nobuaki Sasano

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

The presence of human melanin-concentrating hormone (MCH) was studied in the human brain by radioimmunoassay and immunocytochemistry. Immunoreactive MCH concentrations in the human brain ranged from 0.07 to 19.7 pmol/g wet weight. High performance liquid chromatography of the hypothalamus showed a large immunoreactive peak in the position of human/rat MCH, which was eluted 9 min later than that of salmon MCH. Free-floating sections (40 μm) of the hypothalamus were immunostained. Positive MCH immunostaining was found in perifornical, tuberomammillary, and posterior nuclei. Numerous MCH-immunoreactive nerve fibers were observed throughout the hypothalamus. The presence of high concentrations of MCH in the human brain, in particular in the hypothalamus, suggests that MCH is a neurotransmitter, a neuromodulator, or a neurohormone in man.

Original languageEnglish
Pages (from-to)643-646
Number of pages4
JournalPeptides
Volume14
Issue number3
DOIs
Publication statusPublished - 1993

Keywords

  • Brain
  • Chromatography
  • Human
  • Hypothalamus
  • Immunocytochemistry
  • Melanin-concentrating hormone
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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