Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells

Daisuke Inoue; Mutsuo Yamaya; Hiroshi Kubo; Takahiko Sasaki; Masayoshi Hosoda; Muneo Numasaki; Yoshihisa Tomioka; Hiroyasu Yasuda; Kiyohisa Sekizawa; Hidekazu Nishimura; Hidetada Sasaki

doi:10.1016/j.resp.2005.11.006

Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells

Daisuke Inoue, Mutsuo Yamaya, Hiroshi Kubo, Takahiko Sasaki, Masayoshi Hosoda, Muneo Numasaki, Yoshihisa Tomioka, Hiroyasu Yasuda, Kiyohisa Sekizawa, Hidekazu Nishimura, Hidetada Sasaki

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Mucus hypersecretion relates to exacerbations of bronchial asthma and chronic obstructive pulmonary disease (COPD) caused by rhinovirus (RV) infection. We examined the mechanisms of RV infection-induced mucin production in human tracheal surface epithelial cells and submucosal gland cells. RV14 up-regulated the mRNA expression of MUC2, MUC3, MUC5AC, MUC5B and MUC6, and increased MUC5AC and total mucin concentration in supernatants and lysates of the surface cells. An inhibitor of the nuclear factor κB caffeic acid phenylethyl ester, inhibitors of selective p44/42 mitogen-activated protein kinase-kinase PD98059 and U0126, and a selective Src inhibitor PP1 attenuated MUC5AC mRNA expression, and secretion and production of MUC5AC and total mucin glycoprotein in the surface cells. In the gland cells, RV14 also increased mRNA expression of MUC2, MUC5AC, MUC5B and MUC7, and the inhibitors attenuated the secretion of total mucin glycoprotein. Src-related p44/42 mitogen-activated protein kinase pathway may be associated with RV-induced mucin hypersecretion in human airways.

Original language	English
Pages (from-to)	484-499
Number of pages	16
Journal	Respiratory Physiology and Neurobiology
Volume	154
Issue number	3
DOIs	https://doi.org/10.1016/j.resp.2005.11.006
Publication status	Published - 2006 Dec 1

Keywords

Airway epithelium
MAPK/ERK
Mucin
NF-κB
Rhinovirus

ASJC Scopus subject areas

Neuroscience(all)
Physiology
Pulmonary and Respiratory Medicine

Access to Document

10.1016/j.resp.2005.11.006

Fingerprint Dive into the research topics of 'Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells'. Together they form a unique fingerprint.

Cite this

Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells. / Inoue, Daisuke; Yamaya, Mutsuo; Kubo, Hiroshi; Sasaki, Takahiko; Hosoda, Masayoshi; Numasaki, Muneo ; Tomioka, Yoshihisa; Yasuda, Hiroyasu; Sekizawa, Kiyohisa; Nishimura, Hidekazu; Sasaki, Hidetada.

In: Respiratory Physiology and Neurobiology, Vol. 154, No. 3, 01.12.2006, p. 484-499.

Research output: Contribution to journal › Article › peer-review

Inoue, Daisuke ; Yamaya, Mutsuo ; Kubo, Hiroshi ; Sasaki, Takahiko ; Hosoda, Masayoshi ; Numasaki, Muneo ; Tomioka, Yoshihisa ; Yasuda, Hiroyasu ; Sekizawa, Kiyohisa ; Nishimura, Hidekazu ; Sasaki, Hidetada. / Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells. In: Respiratory Physiology and Neurobiology. 2006 ; Vol. 154, No. 3. pp. 484-499.

@article{cef06f4b1e9e400b857ae10d887a351b,

title = "Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells",

abstract = "Mucus hypersecretion relates to exacerbations of bronchial asthma and chronic obstructive pulmonary disease (COPD) caused by rhinovirus (RV) infection. We examined the mechanisms of RV infection-induced mucin production in human tracheal surface epithelial cells and submucosal gland cells. RV14 up-regulated the mRNA expression of MUC2, MUC3, MUC5AC, MUC5B and MUC6, and increased MUC5AC and total mucin concentration in supernatants and lysates of the surface cells. An inhibitor of the nuclear factor κB caffeic acid phenylethyl ester, inhibitors of selective p44/42 mitogen-activated protein kinase-kinase PD98059 and U0126, and a selective Src inhibitor PP1 attenuated MUC5AC mRNA expression, and secretion and production of MUC5AC and total mucin glycoprotein in the surface cells. In the gland cells, RV14 also increased mRNA expression of MUC2, MUC5AC, MUC5B and MUC7, and the inhibitors attenuated the secretion of total mucin glycoprotein. Src-related p44/42 mitogen-activated protein kinase pathway may be associated with RV-induced mucin hypersecretion in human airways.",

keywords = "Airway epithelium, MAPK/ERK, Mucin, NF-κB, Rhinovirus",

author = "Daisuke Inoue and Mutsuo Yamaya and Hiroshi Kubo and Takahiko Sasaki and Masayoshi Hosoda and Muneo Numasaki and Yoshihisa Tomioka and Hiroyasu Yasuda and Kiyohisa Sekizawa and Hidekazu Nishimura and Hidetada Sasaki",

year = "2006",

month = dec,

day = "1",

doi = "10.1016/j.resp.2005.11.006",

language = "English",

volume = "154",

pages = "484--499",

journal = "Respiratory Physiology and Neurobiology",

issn = "1569-9048",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells

AU - Inoue, Daisuke

AU - Yamaya, Mutsuo

AU - Kubo, Hiroshi

AU - Sasaki, Takahiko

AU - Hosoda, Masayoshi

AU - Numasaki, Muneo

AU - Tomioka, Yoshihisa

AU - Yasuda, Hiroyasu

AU - Sekizawa, Kiyohisa

AU - Nishimura, Hidekazu

AU - Sasaki, Hidetada

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Mucus hypersecretion relates to exacerbations of bronchial asthma and chronic obstructive pulmonary disease (COPD) caused by rhinovirus (RV) infection. We examined the mechanisms of RV infection-induced mucin production in human tracheal surface epithelial cells and submucosal gland cells. RV14 up-regulated the mRNA expression of MUC2, MUC3, MUC5AC, MUC5B and MUC6, and increased MUC5AC and total mucin concentration in supernatants and lysates of the surface cells. An inhibitor of the nuclear factor κB caffeic acid phenylethyl ester, inhibitors of selective p44/42 mitogen-activated protein kinase-kinase PD98059 and U0126, and a selective Src inhibitor PP1 attenuated MUC5AC mRNA expression, and secretion and production of MUC5AC and total mucin glycoprotein in the surface cells. In the gland cells, RV14 also increased mRNA expression of MUC2, MUC5AC, MUC5B and MUC7, and the inhibitors attenuated the secretion of total mucin glycoprotein. Src-related p44/42 mitogen-activated protein kinase pathway may be associated with RV-induced mucin hypersecretion in human airways.

AB - Mucus hypersecretion relates to exacerbations of bronchial asthma and chronic obstructive pulmonary disease (COPD) caused by rhinovirus (RV) infection. We examined the mechanisms of RV infection-induced mucin production in human tracheal surface epithelial cells and submucosal gland cells. RV14 up-regulated the mRNA expression of MUC2, MUC3, MUC5AC, MUC5B and MUC6, and increased MUC5AC and total mucin concentration in supernatants and lysates of the surface cells. An inhibitor of the nuclear factor κB caffeic acid phenylethyl ester, inhibitors of selective p44/42 mitogen-activated protein kinase-kinase PD98059 and U0126, and a selective Src inhibitor PP1 attenuated MUC5AC mRNA expression, and secretion and production of MUC5AC and total mucin glycoprotein in the surface cells. In the gland cells, RV14 also increased mRNA expression of MUC2, MUC5AC, MUC5B and MUC7, and the inhibitors attenuated the secretion of total mucin glycoprotein. Src-related p44/42 mitogen-activated protein kinase pathway may be associated with RV-induced mucin hypersecretion in human airways.

KW - Airway epithelium

KW - MAPK/ERK

KW - Mucin

KW - NF-κB

KW - Rhinovirus

UR - http://www.scopus.com/inward/record.url?scp=33751006156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751006156&partnerID=8YFLogxK

U2 - 10.1016/j.resp.2005.11.006

DO - 10.1016/j.resp.2005.11.006

M3 - Article

C2 - 16377262

AN - SCOPUS:33751006156

VL - 154

SP - 484

EP - 499

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 3

ER -