TY - JOUR
T1 - Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells
AU - Inoue, Daisuke
AU - Yamaya, Mutsuo
AU - Kubo, Hiroshi
AU - Sasaki, Takahiko
AU - Hosoda, Masayoshi
AU - Numasaki, Muneo
AU - Tomioka, Yoshihisa
AU - Yasuda, Hiroyasu
AU - Sekizawa, Kiyohisa
AU - Nishimura, Hidekazu
AU - Sasaki, Hidetada
N1 - Funding Information:
We thank Mr. Grant Crittenden for the English correction and Mr. Akira Ohmi and Ms. Michiko Okamoto, Ms. Minako Tada and Ms. Fusako Chiba for technical assistance. Source of financial support: Grant from The Ministry of Education and Scientific Technique (16590732), and The Ministry of Health, Labour and Welfare (17243601) of the Japanese government to MY.
PY - 2006/12
Y1 - 2006/12
N2 - Mucus hypersecretion relates to exacerbations of bronchial asthma and chronic obstructive pulmonary disease (COPD) caused by rhinovirus (RV) infection. We examined the mechanisms of RV infection-induced mucin production in human tracheal surface epithelial cells and submucosal gland cells. RV14 up-regulated the mRNA expression of MUC2, MUC3, MUC5AC, MUC5B and MUC6, and increased MUC5AC and total mucin concentration in supernatants and lysates of the surface cells. An inhibitor of the nuclear factor κB caffeic acid phenylethyl ester, inhibitors of selective p44/42 mitogen-activated protein kinase-kinase PD98059 and U0126, and a selective Src inhibitor PP1 attenuated MUC5AC mRNA expression, and secretion and production of MUC5AC and total mucin glycoprotein in the surface cells. In the gland cells, RV14 also increased mRNA expression of MUC2, MUC5AC, MUC5B and MUC7, and the inhibitors attenuated the secretion of total mucin glycoprotein. Src-related p44/42 mitogen-activated protein kinase pathway may be associated with RV-induced mucin hypersecretion in human airways.
AB - Mucus hypersecretion relates to exacerbations of bronchial asthma and chronic obstructive pulmonary disease (COPD) caused by rhinovirus (RV) infection. We examined the mechanisms of RV infection-induced mucin production in human tracheal surface epithelial cells and submucosal gland cells. RV14 up-regulated the mRNA expression of MUC2, MUC3, MUC5AC, MUC5B and MUC6, and increased MUC5AC and total mucin concentration in supernatants and lysates of the surface cells. An inhibitor of the nuclear factor κB caffeic acid phenylethyl ester, inhibitors of selective p44/42 mitogen-activated protein kinase-kinase PD98059 and U0126, and a selective Src inhibitor PP1 attenuated MUC5AC mRNA expression, and secretion and production of MUC5AC and total mucin glycoprotein in the surface cells. In the gland cells, RV14 also increased mRNA expression of MUC2, MUC5AC, MUC5B and MUC7, and the inhibitors attenuated the secretion of total mucin glycoprotein. Src-related p44/42 mitogen-activated protein kinase pathway may be associated with RV-induced mucin hypersecretion in human airways.
KW - Airway epithelium
KW - MAPK/ERK
KW - Mucin
KW - NF-κB
KW - Rhinovirus
UR - http://www.scopus.com/inward/record.url?scp=33751006156&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751006156&partnerID=8YFLogxK
U2 - 10.1016/j.resp.2005.11.006
DO - 10.1016/j.resp.2005.11.006
M3 - Article
C2 - 16377262
AN - SCOPUS:33751006156
VL - 154
SP - 484
EP - 499
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
SN - 1569-9048
IS - 3
ER -
