Mechanisms of hormonal regulation of endosperm cap-specific gene expression in tomato seeds

Cristina Martínez-Andújar, Wioletta E. Pluskota, George W. Bassel, Masashi Asahina, Piotr Pupel, Theresa T. Nguyen, Noriko Takeda-Kamiya, David Toubiana, Bing Bai, Ryszard J. Gõrecki, Aaron Fait, Shinjiro Yamaguchi, Hiroyuki Nonogaki

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    The micropylar region of endosperm in a seed, which is adjacent to the radicle tip, is called the 'endosperm cap', and is specifically activated before radicle emergence. This activation of the endosperm cap is a widespread phenomenon among species and is a prerequisite for the completion of germination. To understand the mechanisms of endosperm cap-specific gene expression in tomato seeds, GeneChip analysis was performed. The major groups of endosperm cap-enriched genes were pathogenesis-, cell wall-, and hormone-associated genes. The promoter regions of endosperm cap-enriched genes contained DNA motifs recognized by ethylene response factors (ERFs). The tomato ERF1 (TERF1) and its experimentally verified targets were enriched in the endosperm cap, suggesting an involvement of the ethylene response cascade in this process. The known endosperm cap enzyme endo-β-mannanase is induced by gibberellin (GA), which is thought to be the major hormone inducing endosperm cap-specific genes. The mechanism of endo-β-mannanase induction by GA was also investigated using isolated, embryoless seeds. Results suggested that GA might act indirectly on the endosperm cap. We propose that endosperm cap activation is caused by the ethylene response of this tissue, as a consequence of mechanosensing of the increase in embryonic growth potential by GA action.

    Original languageEnglish
    Pages (from-to)575-586
    Number of pages12
    JournalPlant Journal
    Volume71
    Issue number4
    DOIs
    Publication statusPublished - 2012 Aug 1

    Keywords

    • Solanum lycopersicum
    • embryo
    • endosperm
    • ethylene
    • germination
    • gibberellin
    • seed

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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