Mechanisms of Action of Growth Hormone-Releasing Peptide-2 in Bovine Pituitary Cells

S. G. Roh; M. L. He; N. Matsunaga; S. Hidaka; H. Hidari

doi:10.2527/1997.75102744x

Mechanisms of Action of Growth Hormone-Releasing Peptide-2 in Bovine Pituitary Cells

S. G. Roh, M. L. He, N. Matsunaga, S. Hidaka, H. Hidari

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-βNal-Ala-Trp-D-Phe-Lys-NH₂; GHRP-2) in bovine anterior pituitary primary cell culture. Doses of GHRP-2 from 10^-13 to 10^-7 M increased (P < .05) GH secretion. The GHRP-2 (10^-7 M) and GH-releasing factor (GRF; 10^-7 M) administered together had an additive effect on the release of GH (P < .05). Somatostatin (1 μM) decreased GH secretion in response to GHRP-2 and(or) GRF (P < .05). Secretion of GH in response to GHRP-2 was blocked (P < .01) by a GRF receptor antagonist (.1 μM). Nifedipine (10 μM), a voltage-dependent Ca ²⁺ channel blocker, inhibited (P < .01) GHRP-2-stimulated GH release. The GH release in response to GHRP-2 and 4β-phorbol-12-myristate-13-acetate (10^-7 M), a protein kinase C activator, was additive (P < .01). Forskolin (30 μM), a cAMP elevating agent, further stimulated (P < .01) the GH release in response to GHRP-2. Bovine GH concentrations in culture media were assayed by indirect competitive enzymeimmunoassay. These results showed that GHRP-2 1) stimulates GH secretion from bovine pituitary cells, 2) may partially act via GRF receptor, 3) has GH secretion activity caused by Ca²⁺ influx via Ca²⁺ channels, and 4) may increase GH secretion via protein kinase C and cAMP pathways.

Original language	English
Pages (from-to)	2744-2748
Number of pages	5
Journal	Journal of animal science
Volume	75
Issue number	10
DOIs	https://doi.org/10.2527/1997.75102744x
Publication status	Published - 1997 Oct
Externally published	Yes

Keywords

Cattle
Growth Hormone
Hypothalamic Releasing Hormones
Pituitary Gland

ASJC Scopus subject areas

Food Science
Animal Science and Zoology
Genetics

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title = "Mechanisms of Action of Growth Hormone-Releasing Peptide-2 in Bovine Pituitary Cells",

abstract = "We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-βNal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture. Doses of GHRP-2 from 10-13 to 10-7 M increased (P < .05) GH secretion. The GHRP-2 (10-7 M) and GH-releasing factor (GRF; 10-7 M) administered together had an additive effect on the release of GH (P < .05). Somatostatin (1 μM) decreased GH secretion in response to GHRP-2 and(or) GRF (P < .05). Secretion of GH in response to GHRP-2 was blocked (P < .01) by a GRF receptor antagonist (.1 μM). Nifedipine (10 μM), a voltage-dependent Ca 2+ channel blocker, inhibited (P < .01) GHRP-2-stimulated GH release. The GH release in response to GHRP-2 and 4β-phorbol-12-myristate-13-acetate (10-7 M), a protein kinase C activator, was additive (P < .01). Forskolin (30 μM), a cAMP elevating agent, further stimulated (P < .01) the GH release in response to GHRP-2. Bovine GH concentrations in culture media were assayed by indirect competitive enzymeimmunoassay. These results showed that GHRP-2 1) stimulates GH secretion from bovine pituitary cells, 2) may partially act via GRF receptor, 3) has GH secretion activity caused by Ca2+ influx via Ca2+ channels, and 4) may increase GH secretion via protein kinase C and cAMP pathways.",

keywords = "Cattle, Growth Hormone, Hypothalamic Releasing Hormones, Pituitary Gland",

author = "Roh, {S. G.} and He, {M. L.} and N. Matsunaga and S. Hidaka and H. Hidari",

year = "1997",

month = oct,

doi = "10.2527/1997.75102744x",

language = "English",

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pages = "2744--2748",

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AU - Roh, S. G.

AU - He, M. L.

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AU - Hidaka, S.

AU - Hidari, H.

PY - 1997/10

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AB - We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-βNal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture. Doses of GHRP-2 from 10-13 to 10-7 M increased (P < .05) GH secretion. The GHRP-2 (10-7 M) and GH-releasing factor (GRF; 10-7 M) administered together had an additive effect on the release of GH (P < .05). Somatostatin (1 μM) decreased GH secretion in response to GHRP-2 and(or) GRF (P < .05). Secretion of GH in response to GHRP-2 was blocked (P < .01) by a GRF receptor antagonist (.1 μM). Nifedipine (10 μM), a voltage-dependent Ca 2+ channel blocker, inhibited (P < .01) GHRP-2-stimulated GH release. The GH release in response to GHRP-2 and 4β-phorbol-12-myristate-13-acetate (10-7 M), a protein kinase C activator, was additive (P < .01). Forskolin (30 μM), a cAMP elevating agent, further stimulated (P < .01) the GH release in response to GHRP-2. Bovine GH concentrations in culture media were assayed by indirect competitive enzymeimmunoassay. These results showed that GHRP-2 1) stimulates GH secretion from bovine pituitary cells, 2) may partially act via GRF receptor, 3) has GH secretion activity caused by Ca2+ influx via Ca2+ channels, and 4) may increase GH secretion via protein kinase C and cAMP pathways.

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KW - Growth Hormone

KW - Hypothalamic Releasing Hormones

KW - Pituitary Gland

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JO - Journal of Animal Science

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SN - 0021-8812

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Mechanisms of Action of Growth Hormone-Releasing Peptide-2 in Bovine Pituitary Cells

Abstract

Keywords

ASJC Scopus subject areas

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