Mechanism of the down-regulation of cAMP receptor protein by glucose in Escherichia coli: Role of autoregulation of the crp gene

Hitoshi Ishizuka, Akemi Hanamura, Toshifumi Inada, Hiroji Aiba

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Glucose causes catabolite repression by lowering the intracellular levels of both cAMP and cAMP receptor protein (CRP) in Escherichia coli. The molecular mechanism underlying the down-regulation of CRP by glucose has been investigated. We show that glucose lowers the level of crp mRNA without affecting its stability. Replacement of the crp promoter with the bla promoter almost completely abolishes the glucosemediated regulation of crp expression. Only a slight reduction in the crp expression by glucose is observed in cya- or crp- strains, suggesting that a CRP-cAMP complex is needed for this regulation. We previously showed that transcription of the crp gene is regulated both negatively and positively. Positive autoregulation of crp is caused by the binding of CRP-cAMP to the CRP binding site II located upstream of the crp promoter. Here we show that disrupting the CRP binding site II essentially eliminates the down-regulation of crp expression by glucose. We conclude that the autoregulatory circuit of the crp gene plays a key role in the down-regulation of CRP by glucose.

Original languageEnglish
Pages (from-to)3077-3082
Number of pages6
JournalEMBO Journal
Volume13
Issue number13
DOIs
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • Autoregulation
  • CRP-cAMP
  • Catabolite repression
  • Transcriptional activation
  • crp expression

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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