Mechanism of sequential order determination in bio-raman correlation analysis

Sota Takanezawa, Yasushi Sako, Yukihiro Ozaki, Shin Ichi Morita

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Using the measured Raman spectra of triolein and cholesteryl linolenate, the contradiction caused in determining the sequential orders in the two-dimensional correlation spectroscopy was exemplified, in which time-profiles of four marker bands A, B, C, and D were modeled so that A→B→C→ D. Here 'A→B' is such notation that we read as 'A is occurred before B' or 'A earlier than B'. The two-dimensional correlation method gave the result B→C→D→A which was contradictive to the initial setting. We confirmed that the increments of distance between the peak positions of the Gaussian type time-profiles f and g, through a threshold, gave the unexpected switch in the sequential order. On the complex plane based on the synchronous and asynchronous axes, the vector g is identical to the synchronous axis in direction; the vector f is crossed through the asynchronous axis corresponding to the increments of distance between the peak positions of f and g. By the conventional rule of the correlation method, the vector f just crossing the asynchronous axis is not allowed to be in the second quadrant, but the vector f is transformed to the fourth quadrant with 180 degrees shifted. In the situation, the vector f is located in the later position for the standard vector g, that is, f is occurred 'after' g. Based on the revealed mechanism for the contradiction, the time series correlation analysis may be extended in a more versatile manner to allow bio-Raman correlation analysis on diverse dynamics of bio-molecules in living cells.

Original languageEnglish
Title of host publicationNano-Bio Sensing, Imaging, and Spectroscopy
DOIs
Publication statusPublished - 2013 Sep 17
Externally publishedYes
EventSPIE Nano-Bio Sensing, Imaging, and Spectroscopy - Jeju, Korea, Republic of
Duration: 2013 Feb 202013 Feb 23

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8879
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceSPIE Nano-Bio Sensing, Imaging, and Spectroscopy
CountryKorea, Republic of
CityJeju
Period13/2/2013/2/23

Keywords

  • Raman spectroscopy
  • sequential order
  • two-dimensional correlation spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Mechanism of sequential order determination in bio-raman correlation analysis'. Together they form a unique fingerprint.

Cite this