Mechanism of mesoconitine-induced contractile response in guinea pig vas deferens

Hiroshi Sato, Yasushi Ohizumi, Hiroshi Hikino

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Mesaconitine (MA) caused contraction of the isolated guinea pig vas deferens in a dose-dependent manner (3 × 10-6-10-4 g/ml) and nonspecifically potentiated the contractile response to norepinephrine (NE), acetylcholine, histamine and tyramine. Tachyphylaxis developed during the repeated application of MA. The contraction produced by MA was prevented by treatment with phentolamine, phenoxybenzamine, procaine, bretylium or tetrodotoxin, pretreatment with reserpine or 6-hydroxydopamine, or denervation. Atropine or hexamethonium did not abolish the effect of MA, excluding the possibility that the muscarinic and nicotinic receptors are the site of action of MA. MA elicited a dose-dependent release of NE from the isolated vas deferens which had been blocked by treatment with tetrodotoxin, pretreatment with reserpine, or the exclusion of calcium ion from the bath medium. Consequently, it is concluded that MA-induced contraction was brought about by the release of NE from pre-synaptic neuronal sites and by excitation of the post-synaptic membrane.

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalEuropean Journal of Pharmacology
Volume55
Issue number1
DOIs
Publication statusPublished - 1979 Apr 1

Keywords

  • Guinea pig vas deferens
  • Mesaconitine
  • Norepinephrine release
  • Postsynaptic action of mesaconitine
  • Presynaptic action of mesaconitine
  • Tachyphylaxis

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Mechanism of mesoconitine-induced contractile response in guinea pig vas deferens'. Together they form a unique fingerprint.

Cite this