Mechanism of Cell Damage by Ultrasound in Combination with Hematoporphyrin

Shin‐ichiro ‐i Umemura, Nagahiko Yumita, Ryuichiro Nishigaki, Koshiro Umemura

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257 Citations (Scopus)


The mechanism of cell damage by ultrasound in combination with hematoporphyrin was studied. Mouse sarcoma 180 cell suspensions were exposed to ultrasound for up to 60 s in the presence and absence of hematoporphyrin, with and without active oxygen scavengers. The cell damage enhancement by hematoporphyrin was suppressed by adding histidine but not by mannitol. The enhancement was doubled in rate by substitution of deuterium oxide medium for normal water. Sonoluminescence was produced in a saline solution under similar acoustic conditions and observed to have spectral components that can excite hematoporphyrin molecules. These results suggest that cell damage enhancement is probably mediated via singlet oxygen generated by ultrasonically activated hematoporphyrin.

Original languageEnglish
Pages (from-to)962-966
Number of pages5
JournalJapanese Journal of Cancer Research
Issue number9
Publication statusPublished - 1990 Sep
Externally publishedYes


  • Active oxygen scavenger
  • Cell damage
  • Hematoporphyrin
  • Singlet oxygen
  • Ultrasound

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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