The effects of a novel anti-cancer drug, YM 534 on human promyelocytic leukemia line HL-60 cells were investigated. The growth of the cells was completely inhibited with an IC50 of 2.5 x 10-6 M. The incorporation of 3H-thymidine or 3H-uridine into acid insoluble fraction of the cells was completely inhibited at a 10-4 M concentration of the drug. However, that of 3H-leucine was suppressed by only 32% at this drug concentration. The single strand scission of DNA of the cells as revealed by alkali sucrose density gradient centrifugation was induced by YM 534 at a 10-4 M concentration for 60 min. When PM2 DNA in a cell-free system was examined in the presence or absence of reducing agent, no damage to DNA was observed at a 10-4 M concentration of YM 534. The processing of preribosomal to ribosomal RNA of HL-60 cells was retarded at a 10-5 M level of the drug.
|Number of pages||8|
|Journal||Anti-Cancer Drug Design|
|Publication status||Published - 1989 Jan 1|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Drug Discovery
- Organic Chemistry