Mechanism of action of the synthetic imidazole compound YM 534 on human promyelocytic leukemia line HL-60 cells

R. Kanamaru; Chikashi Ishioka; Y. Konishi; A. Ishikawa; H. Shibata; A. Wakui

Mechanism of action of the synthetic imidazole compound YM 534 on human promyelocytic leukemia line HL-60 cells

R. Kanamaru, Chikashi Ishioka, Y. Konishi, A. Ishikawa, H. Shibata, A. Wakui

Division of Cancer Science

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The effects of a novel anti-cancer drug, YM 534 on human promyelocytic leukemia line HL-60 cells were investigated. The growth of the cells was completely inhibited with an IC₅₀ of 2.5 x 10^-6 M. The incorporation of ³H-thymidine or ³H-uridine into acid insoluble fraction of the cells was completely inhibited at a 10^-4 M concentration of the drug. However, that of ³H-leucine was suppressed by only 32% at this drug concentration. The single strand scission of DNA of the cells as revealed by alkali sucrose density gradient centrifugation was induced by YM 534 at a 10^-4 M concentration for 60 min. When PM2 DNA in a cell-free system was examined in the presence or absence of reducing agent, no damage to DNA was observed at a 10^-4 M concentration of YM 534. The processing of preribosomal to ribosomal RNA of HL-60 cells was retarded at a 10^-5 M level of the drug.

Original language	English
Pages (from-to)	137-144
Number of pages	8
Journal	Anti-Cancer Drug Design
Volume	4
Issue number	2
Publication status	Published - 1989 Jan 1

ASJC Scopus subject areas

Biochemistry
Biochemistry, Genetics and Molecular Biology(all)
Oncology
Pharmacology
Drug Discovery
Organic Chemistry

Access to Document

Fingerprint Dive into the research topics of 'Mechanism of action of the synthetic imidazole compound YM 534 on human promyelocytic leukemia line HL-60 cells'. Together they form a unique fingerprint.

Cite this

@article{09db3206a8a743298cc218e5152cb2e7,

title = "Mechanism of action of the synthetic imidazole compound YM 534 on human promyelocytic leukemia line HL-60 cells",

abstract = "The effects of a novel anti-cancer drug, YM 534 on human promyelocytic leukemia line HL-60 cells were investigated. The growth of the cells was completely inhibited with an IC50 of 2.5 x 10-6 M. The incorporation of 3H-thymidine or 3H-uridine into acid insoluble fraction of the cells was completely inhibited at a 10-4 M concentration of the drug. However, that of 3H-leucine was suppressed by only 32% at this drug concentration. The single strand scission of DNA of the cells as revealed by alkali sucrose density gradient centrifugation was induced by YM 534 at a 10-4 M concentration for 60 min. When PM2 DNA in a cell-free system was examined in the presence or absence of reducing agent, no damage to DNA was observed at a 10-4 M concentration of YM 534. The processing of preribosomal to ribosomal RNA of HL-60 cells was retarded at a 10-5 M level of the drug.",

author = "R. Kanamaru and Chikashi Ishioka and Y. Konishi and A. Ishikawa and H. Shibata and A. Wakui",

year = "1989",

month = jan,

day = "1",

language = "English",

volume = "4",

pages = "137--144",

journal = "Anti-Cancer Drug Design",

issn = "0266-9536",

publisher = "Cognizant Communication Corporation",

number = "2",

}

TY - JOUR

T1 - Mechanism of action of the synthetic imidazole compound YM 534 on human promyelocytic leukemia line HL-60 cells

AU - Kanamaru, R.

AU - Ishioka, Chikashi

AU - Konishi, Y.

AU - Ishikawa, A.

AU - Shibata, H.

AU - Wakui, A.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The effects of a novel anti-cancer drug, YM 534 on human promyelocytic leukemia line HL-60 cells were investigated. The growth of the cells was completely inhibited with an IC50 of 2.5 x 10-6 M. The incorporation of 3H-thymidine or 3H-uridine into acid insoluble fraction of the cells was completely inhibited at a 10-4 M concentration of the drug. However, that of 3H-leucine was suppressed by only 32% at this drug concentration. The single strand scission of DNA of the cells as revealed by alkali sucrose density gradient centrifugation was induced by YM 534 at a 10-4 M concentration for 60 min. When PM2 DNA in a cell-free system was examined in the presence or absence of reducing agent, no damage to DNA was observed at a 10-4 M concentration of YM 534. The processing of preribosomal to ribosomal RNA of HL-60 cells was retarded at a 10-5 M level of the drug.

AB - The effects of a novel anti-cancer drug, YM 534 on human promyelocytic leukemia line HL-60 cells were investigated. The growth of the cells was completely inhibited with an IC50 of 2.5 x 10-6 M. The incorporation of 3H-thymidine or 3H-uridine into acid insoluble fraction of the cells was completely inhibited at a 10-4 M concentration of the drug. However, that of 3H-leucine was suppressed by only 32% at this drug concentration. The single strand scission of DNA of the cells as revealed by alkali sucrose density gradient centrifugation was induced by YM 534 at a 10-4 M concentration for 60 min. When PM2 DNA in a cell-free system was examined in the presence or absence of reducing agent, no damage to DNA was observed at a 10-4 M concentration of YM 534. The processing of preribosomal to ribosomal RNA of HL-60 cells was retarded at a 10-5 M level of the drug.

UR - http://www.scopus.com/inward/record.url?scp=0024415884&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024415884&partnerID=8YFLogxK

M3 - Article

C2 - 2803462

AN - SCOPUS:0024415884

VL - 4

SP - 137

EP - 144

JO - Anti-Cancer Drug Design

JF - Anti-Cancer Drug Design

SN - 0266-9536

IS - 2

ER -