Abstract
The effects of a novel anti-cancer drug, YM 534 on human promyelocytic leukemia line HL-60 cells were investigated. The growth of the cells was completely inhibited with an IC50 of 2.5 x 10-6 M. The incorporation of 3H-thymidine or 3H-uridine into acid insoluble fraction of the cells was completely inhibited at a 10-4 M concentration of the drug. However, that of 3H-leucine was suppressed by only 32% at this drug concentration. The single strand scission of DNA of the cells as revealed by alkali sucrose density gradient centrifugation was induced by YM 534 at a 10-4 M concentration for 60 min. When PM2 DNA in a cell-free system was examined in the presence or absence of reducing agent, no damage to DNA was observed at a 10-4 M concentration of YM 534. The processing of preribosomal to ribosomal RNA of HL-60 cells was retarded at a 10-5 M level of the drug.
| Original language | English |
|---|---|
| Pages (from-to) | 137-144 |
| Number of pages | 8 |
| Journal | Anti-Cancer Drug Design |
| Volume | 4 |
| Issue number | 2 |
| Publication status | Published - 1989 |
ASJC Scopus subject areas
- Biochemistry
- Oncology
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology
- Drug Discovery
- Organic Chemistry
