Mechanism of action of the synthetic imidazole compound YM 534 on human promyelocytic leukemia line HL-60 cells

R. Kanamaru, C. Ishioka, Y. Konishi, A. Ishikawa, H. Shibata, A. Wakui

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1 Citation (Scopus)

Abstract

The effects of a novel anti-cancer drug, YM 534 on human promyelocytic leukemia line HL-60 cells were investigated. The growth of the cells was completely inhibited with an IC50 of 2.5 x 10-6 M. The incorporation of 3H-thymidine or 3H-uridine into acid insoluble fraction of the cells was completely inhibited at a 10-4 M concentration of the drug. However, that of 3H-leucine was suppressed by only 32% at this drug concentration. The single strand scission of DNA of the cells as revealed by alkali sucrose density gradient centrifugation was induced by YM 534 at a 10-4 M concentration for 60 min. When PM2 DNA in a cell-free system was examined in the presence or absence of reducing agent, no damage to DNA was observed at a 10-4 M concentration of YM 534. The processing of preribosomal to ribosomal RNA of HL-60 cells was retarded at a 10-5 M level of the drug.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalAnti-Cancer Drug Design
Volume4
Issue number2
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Biochemistry
  • Oncology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

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