Mechanical characterization of cyanobacteria under osmotic stress

S. Sahuma, D. Chang, F. Arai, K. Kera, N. Uozumi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the evaluation results of mechanical characterization of Synechocystis sp. PCC 6803. We have constructed the force measurement system using the microfluidic chip on the microscope with optical tweezers. Indentation and force sensor probes are integrated into the microfluidic chip. Using the constructed system, we evaluated the Young's modulus of two cell groups of wild type and mutant type, which was knocked out mechanosensitive channels. They are placed in two different mediums; normal and high-osmolality condition. While there was no significant difference of stiffness between two groups in normal medium, the average value and the standard deviation of Young's modulus were different in high-osmolality condition. These results indicate the basic function of mechanosensitive channels, because there was clear relationship between the stiffness and the osmotic stress. We confirmed that the measurement system will contribute to clarify unknown function of ion channels of cyanobacteria based on relationship between environmental stress and mechanical characteristics on single cell level.

Original languageEnglish
Title of host publication2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1248-1251
Number of pages4
ISBN (Electronic)9781509050789
DOIs
Publication statusPublished - 2017 Feb 23
Event30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 - Las Vegas, United States
Duration: 2017 Jan 222017 Jan 26

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
CountryUnited States
CityLas Vegas
Period17/1/2217/1/26

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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