Measurements of strain on single stress fibers in living endothelial cells induced by fluid shear stress

Yosuke Ueki, Yuhei Uda, Naoya Sakamoto, Masaaki Sato

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Fluid shear stress (FSS) acting on the apical surface of endothelial cells (ECs) can be sensed by mechano-sensors in adhesive protein complexes found in focal adhesions and intercellular junctions. This sensing occurs via force transmission through cytoskeletal networks. This study quantitatively evaluated the force transmitted through cytoskeletons to the mechano-sensors by measuring the FSS-induced strain on SFs using live-cell imaging for actin stress fibers (SFs). FSS-induced bending of SFs caused the SFs to align perpendicular to the direction of the flow. In addition, the displacement vectors of the SFs were detected using image correlation and the FSS-induced axial strain of the SFs was calculated. The results indicated that FSS-induced strain on SFs spanned the range 0.01-0.1% at FSSs ranging from 2 to 10 Pa. Together with the tensile property of SFs reported in a previous study, the force exerted on SFs was estimated to range from several to several tens of pN.

Original languageEnglish
Pages (from-to)441-446
Number of pages6
JournalBiochemical and biophysical research communications
Volume395
Issue number3
DOIs
Publication statusPublished - 2010 May 7

Keywords

  • Cytoskeleton
  • Endothelial cell
  • Fluid shear stress
  • Force transmission
  • Stress fiber

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Measurements of strain on single stress fibers in living endothelial cells induced by fluid shear stress'. Together they form a unique fingerprint.

Cite this