Measurement of the molecular mass dependence of the mass diffusion coefficient in protein aqueous solutions

Juan F. Torres, Atsuki Komiya, Junnosuke Okajima, Maruyama Shigenao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This paper reports the measurement of the binary mass diffusion coefficient for proteins with a wide range of molecular size. The diffusion coefficient is obtained by conducting diffusion experiments in the dilute region. Transient concentration profiles were measured by a phase-shifting interferometer and subsequently compared with a numerical calculation based on Fick's law to determine the diffusion coefficient. Distilled water was used as solvent in free diffusion experiments conducted at T = (25 ± 1.0)°C. The method was validated by measuring the diffusion coefficient of aqueous NaCl, Sucrose, and BSA, which values have been extensively reported in the literature. The values of the diffusion coefficient for seven proteins: aprotinin (6.5 kDa), α-lactalbumin (14.2 kDa), lysozyme (14.3 kDa), trypsin inhibitor (20.1 kDa), ovalbumin (44.2 kDa), bovine serum albumin (66.7 kDa), and phosphorylase b (97.2 kDa), were determined in the dilute region of 0-3 mg/ml. The results are compared with the Stokes-Einstein equation. The influence of the molecular structure and pH on the diffusion coefficient is discussed.

Original languageEnglish
Title of host publicationDiffusion in Solids and Liquids VII
Subtitle of host publicationSolids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials
PublisherTrans Tech Publications Ltd
Pages452-458
Number of pages7
Volume326-328
ISBN (Print)9783037854006
DOIs
Publication statusPublished - 2012 Jan 1
Event7th International Conference on Diffusion in Solids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials, DSL 2011 - Algarve, Portugal
Duration: 2011 Jun 262011 Jun 30

Other

Other7th International Conference on Diffusion in Solids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials, DSL 2011
CountryPortugal
CityAlgarve
Period11/6/2611/6/30

Keywords

  • Diffusion coefficient
  • Molecular mass
  • Phase-shifting interferometer
  • Protein

ASJC Scopus subject areas

  • Radiation
  • Materials Science(all)
  • Condensed Matter Physics

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  • Cite this

    Torres, J. F., Komiya, A., Okajima, J., & Shigenao, M. (2012). Measurement of the molecular mass dependence of the mass diffusion coefficient in protein aqueous solutions. In Diffusion in Solids and Liquids VII: Solids and Liquids, Mass Transfer - Heat Transfer - Microstructure and Properties - Nanodiffusion and Nanostructured Materials (Vol. 326-328, pp. 452-458). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/DDF.326-328.452