Measurement of Srs-A Activities in Plasma of Asthmatic Patients

Hiroshi Otsu, Suetsugu Mue, Gen Tamura, Kohei Yamauchi, Toshiharu Ishihara, Kohichi Sato, Tamotsu Takishima

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5 Citations (Scopus)


Ethanol extracts of arterial blood from 20 of 29 asthmatic patients during attacks produced sustained contractions of the guinea pig ileum. These contractions were inhibited by FPL 55712, a specific antagonist of slow reacting substance of anaphylaxis (SRS-A). SRS-A activity was significantly higher during attack than during remission. Reverse-phase high pressure liquid chromatography (HPLC) was performed on two extracts which had shown high SRS-A activity with bioassay. One extract showed double peaks, which had retention times identical to those of leukotriene (LT) C4 and LTD4. The other extract showed a single peak, which had a retention time identical to that of synthetic LTC4. bronchial asthma; slow reacting substance of anaphylaxis (SRS-A) ; bioassay ; high pressure liquid chromatography (HPLC).

Original languageEnglish
Pages (from-to)197-203
Number of pages7
Journalthe tohoku journal of experimental medicine
Issue number2
Publication statusPublished - 1985 Jan 1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Otsu, H., Mue, S., Tamura, G., Yamauchi, K., Ishihara, T., Sato, K., & Takishima, T. (1985). Measurement of Srs-A Activities in Plasma of Asthmatic Patients. the tohoku journal of experimental medicine, 145(2), 197-203.