Measurement of serum PSP/reg-protein concentration in various diseases with a newly developed enzyme-linked immunosorbent assay

Yoshitake Satomura, Norio Sawabu, Ikurou Mouri, Osamu Yamakawa, Hiroyuki Watanabe, Yoshiharu Motoo, Takashi Okai, Takako Ito, Kazue Kaneda, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay, based on two monoclonal antibodies (H reg1-1 and H reg101-1) specific for pancreatic stone protein (PSP)/reg-protein, was developed to determine the concentration of this protein in serum from individuals with various diseases. The serum concentration of PSP/reg-protein was significantly higher in patients with various pancreatic diseases than in normal controls, and was also significantly higher in patients with acute pancreatitis or chronic relapsing pancreatitis than in patients with chronic pancreatitis. Furthermore, the serum PSP/reg-protein concentration was also significantly increased in liver cirrhosis, choledocholithiasis, and various cancers of the digestive system, and was extremely high in all patients tested with chronic renal failure. A significant correlation was apparent between the serum concentration of PSP/reg-protein and elastase-I in 68 patients with chronic pancreatitis or pancreatic cancer. Whereas only 7 of these patients showed a normal serum PSP/reg-protein concentration and a significantly increased elastase-I concentration, 15 of these patients showed a significantly increased serum PSP/reg-protein coecentration and a normal serum elastase-1 concentration. These results indicate that the serum PSP/reg-protein concentration may reflect pancreatic damage, especially in acute pancreatitis, and may be as sensitive a marker for such damage as elastase-I, although false positivity was apparent in renal failure and in some patients with hepatic dysfunction or digestive system malignancies.

Original languageEnglish
Pages (from-to)643-650
Number of pages8
JournalJournal of gastroenterology
Volume30
Issue number5
DOIs
Publication statusPublished - 1995 Oct

Keywords

  • elastase-I
  • enzyme-linked immunosorbent assay (ELISA)
  • pancreatic stone protein (PSP)
  • pancreatitis
  • reg-protein

ASJC Scopus subject areas

  • Gastroenterology

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