Measurement of lysophospholipase D/autotaxin activity in human serum samples

Kazuhiro Nakamura, Ryunosuke Ohkawa, Shigeo Okubo, Minoru Tozuka, Mitsumasa Okada, Shinya Aoki, Junken Aoki, Hiroyuki Arai, Hitoshi Ikeda, Yutaka Yatomi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Objectives: Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing. Design and methods: LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate. Results and conclusions: It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.

Original languageEnglish
Pages (from-to)274-277
Number of pages4
JournalClinical Biochemistry
Volume40
Issue number3-4
DOIs
Publication statusPublished - 2007 Feb 1
Externally publishedYes

Keywords

  • Autotaxin
  • Lysophosphatidic acid
  • Lysophosphatidylcholine
  • Lysophospholipase D
  • Plasma

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Nakamura, K., Ohkawa, R., Okubo, S., Tozuka, M., Okada, M., Aoki, S., Aoki, J., Arai, H., Ikeda, H., & Yatomi, Y. (2007). Measurement of lysophospholipase D/autotaxin activity in human serum samples. Clinical Biochemistry, 40(3-4), 274-277. https://doi.org/10.1016/j.clinbiochem.2006.10.009