TY - JOUR
T1 - Measurement of individual cell strength of Botryococcus braunii in cell culture
AU - Tsutsumi, Shun
AU - Saito, Yasuhiro
AU - Matsushita, Yohsuke
AU - Aoki, Hideyuki
N1 - Funding Information:
Funding information This work was supported by the Next-Generation Energies for Tohoku Recovery (NET) project of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan. The samples of B. braunii were provided by the University of Tsukuba. The cell compression test using nanoindentation was supported by the Kyoto University Nano Technology Hub in BNanotechnology Platform Project^ sponsored by MEXT, Japan.
Publisher Copyright:
© 2018, Springer Science+Business Media B.V., part of Springer Nature.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Botryococcus braunii is a microalga considered for biofuel production and may require physical disruption of cells/colonies for efficient hydrocarbon extraction. In this study, the strength of individual cells of B. braunii was measured using a nanoindenter. From the load and cell size, the pressure for bursting the cell was calculated to be 56.9 MPa. This value is 2.3–10 times those of Saccharomyces cerevisiae and Chlorella vulgaris found in another research, because B. braunii has two types of cell walls with different thicknesses. The energy required to disrupt 1 g of dry B. braunii cells, estimated by load-displacement curves, is 3.19 J g−1 which is 0.19–1.2 times higher than those of S. cerevisiae and C. vulgaris. When using a high-pressure homogenizer for disrupting B. braunii cells, the cell disruption degree increased with the treatment pressure at above 30 MPa, and 70% of cells were disrupted at 80 MPa.
AB - Botryococcus braunii is a microalga considered for biofuel production and may require physical disruption of cells/colonies for efficient hydrocarbon extraction. In this study, the strength of individual cells of B. braunii was measured using a nanoindenter. From the load and cell size, the pressure for bursting the cell was calculated to be 56.9 MPa. This value is 2.3–10 times those of Saccharomyces cerevisiae and Chlorella vulgaris found in another research, because B. braunii has two types of cell walls with different thicknesses. The energy required to disrupt 1 g of dry B. braunii cells, estimated by load-displacement curves, is 3.19 J g−1 which is 0.19–1.2 times higher than those of S. cerevisiae and C. vulgaris. When using a high-pressure homogenizer for disrupting B. braunii cells, the cell disruption degree increased with the treatment pressure at above 30 MPa, and 70% of cells were disrupted at 80 MPa.
KW - Botryococcus braunii
KW - Cell disruption
KW - Cell strength
KW - Microalgae
KW - Nanoindentation
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U2 - 10.1007/s10811-018-1466-6
DO - 10.1007/s10811-018-1466-6
M3 - Article
AN - SCOPUS:85051056196
VL - 30
SP - 2287
EP - 2296
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
SN - 0921-8971
IS - 4
ER -