Measurement of Forces between Surfaces Composed of Two-Dimensionally Organized, Complementary and Noncomplementary Nucleobases

Peter Berndt, Kazue Kurihara, Toyoki Kunitake

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Langmuir-Blodgett films of amphiphiles with an orotic acid (Oro, uracil-like) headgroup or a complementary adenine (Ade) headgroup were deposited onto mica surfaces and investigated by surface force measurement. Interactions between both complementary (Oro-Ade) and noncomplementary (Ade-Ade, Oro-Oro) nucleobase pairs were studied. The LB films showed functional features of the nucleobase headgroups. Electrolyte-dependent electrostatic repulsion was found at pH's close to the pKa of the headgroups. In the absence of strong electrostatic forces, i.e., Oro-Ade and Oro-Oro combinations at pH 5.6, weak long-range attraction was detected. At close distances strong attractive forces made the surfaces jump into a contact position. The adhesive force between the complementary layers was found to be 100 mN/m, while the values between the noncomplementary pairs were ca. 50 mN/m and 250 ± 50 mN/m for Ade-Ade and Oro-Oro, respectively. The force profiles found between nucleobase surfaces are made of two components. One is observed at ca. 100-20 nm and changes its intensity drastically depending on conditions. This force is found both for complementary and for noncomplementary surfaces and is similar to the very long range attraction reported for hydrophobic surfaces (Claesson et al., 1988, 1994; Kurihara et al., 1990, 1992; Evans, 1993). The other, appearing at around 20 nm, is characteristic of complementary pairs and remains unchanged for pH changes and by salt (KBr) addition. These forces may have an important bearing on interaction mechanisms involved in molecular recognition and supramolecular assembling in biological systems.

Original languageEnglish
Pages (from-to)3083-3091
Number of pages9
JournalLangmuir
Volume11
Issue number8
DOIs
Publication statusPublished - 1995 Aug 1
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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