Measurement of density and affinity for dopamine D 2 receptors by a single positron emission tomography scan with multiple injections of 11 Craclopride

Yoko Ikoma, Hiroshi Watabe, Takuya Hayashi, Yoshinori Miyake, Noboru Teramoto, Kotaro Minato, Hidehiro Iida

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Positron emission tomography (PET) with 11 Craclopride has been used to investigate the density (Bmax) and affinity (Kd) of dopamine D2 receptors related to several neurological and psychiatric disorders. However, in assessing the Bmax and Kd, multiple PET scans are necessary under variable specific activities of administered 11 Craclopride, resulting in a long study period and unexpected physiological variations. In this paper, we have developed a method of multiple-injection graphical analysis (MI-GA) that provides the Bmax and Kd values from a single PET scan with three sequential injections of 11 Craclopride, and we validated the proposed method by performing numerous simulations and PET studies on monkeys. In the simulations, the three-injection protocol was designed according to prior knowledge of the receptor kinetics, and the errors of Bmax and K d estimated by MI-GA were analyzed. Simulations showed that our method could support the calculation of B max and Kd, despite a slight overestimation compared with the true magnitudes. In monkey studies, we could calculate the Bmax and Kd of diseased or normal striatum in a 150 mins scan with the three-injection protocol of 11 Craclopride. Estimated Bmax and K d values of D2 receptors in normal or partially dopamine-depleted striatum were comparable to the previously reported values.

Original languageEnglish
Pages (from-to)663-673
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume30
Issue number3
DOIs
Publication statusPublished - 2010 Mar
Externally publishedYes

Keywords

  • Dopamine D2 receptors
  • Graphical analysis
  • Multiple injections
  • Positron emission tomography
  • [C]raclopride

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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