Measurement of abscisic acid and gibberellins by gas chromatography/mass spectrometry

Masanori Okamoto, Atsushi Hanada, Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara

    Research output: Chapter in Book/Report/Conference proceedingChapter

    14 Citations (Scopus)

    Abstract

    Gas chromatography-mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.

    Original languageEnglish
    Title of host publicationPlant Hormones
    Subtitle of host publicationMethods and Protocols
    PublisherHumana Press
    Pages53-60
    Number of pages8
    ISBN (Print)9781934115329
    DOIs
    Publication statusPublished - 2009

    Publication series

    NameMethods in Molecular Biology
    Volume495
    ISSN (Print)1064-3745

    Keywords

    • Abscisic acid
    • Derivatization
    • Gas chromatography-mass spectrometry (GC-MS)
    • Gibberellins
    • Internal standard

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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