MC3T3-E1 and RAW264.7 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin

Masakazu Kawashita, Jumpei Hayashi, Tada Aki Kudo, Hiroyasu Kanetaka, Zhixia Li, Toshiki Miyazaki, Masami Hashimoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2O3, but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al 2O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2O3. Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity.

Original languageEnglish
Pages (from-to)1880-1886
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number6
DOIs
Publication statusPublished - 2014 Jun

Keywords

  • MC3T3-E1
  • RAW264.7
  • alumina
  • bovine serum albumin
  • hydroxyapatite

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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