MC3T3-E1 and RAW264.7 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin

Masakazu Kawashita, Jumpei Hayashi, Tada Aki Kudo, Hiroyasu Kanetaka, Zhixia Li, Toshiki Miyazaki, Masami Hashimoto

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2O3, but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al 2O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2O3. Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity.

Original languageEnglish
Pages (from-to)1880-1886
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number6
DOIs
Publication statusPublished - 2014 Jun

Keywords

  • MC3T3-E1
  • RAW264.7
  • alumina
  • bovine serum albumin
  • hydroxyapatite

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Fingerprint

Dive into the research topics of 'MC3T3-E1 and RAW264.7 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin'. Together they form a unique fingerprint.

Cite this