MAT-1, a monoclonal antibody that specifically recognizes human tyrosinase

H. Takimoto, S. Suzuki, S. Masui, K. Shibata, Y. Tomita, S. Shibahara, H. Nakano

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

There is no available monoclonal antibody which reacts specifically recognizes human tyrosinase. Employing a synthetic peptide, MEKEDYHSLYQSHL, corresponding to the carboxyl terminus of human tyrosinase as an immunogen, we produced a mouse monoclonal antibody MAT-1 of the IgG1 isotype. The epitope for MAT-1 was determined to be EDYH, the sequence of which is not present in human tyrosinase-related protein-1 (TRP-1) or tyrosinase-related protein-2 (TRP-2). By transient expression assays and immunofluorescence technique, we show that MAT-1 reacts specifically with cells expressing human tyrosinase cDNA but not with cells expressing TRP-1 or TRP-2 cDNA. The results of immunohistochemical staining also confirmed that MAT-1 reacts specifically with epidermal melanocytes in human skin sections. MAT-1 should be invaluable for studying the interaction between tyrosinase and TRPs and for detecting the changes in the levels of tyrosinase expression. In addition, MAT-1 should be useful as a sensitive immunohistochemical tool for investigation of various pigmentary disorders and possibly for the diagnosis of melanoma.

Original languageEnglish
Pages (from-to)764-768
Number of pages5
JournalJournal of Investigative Dermatology
Volume105
Issue number6
DOIs
Publication statusPublished - 1995

Keywords

  • Melanin
  • Melanocyte
  • Pigmentation
  • cDNA-transfected cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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