Mapping peroxidase in plant tissues by scanning electrochemical microscopy

Huafang Zhou, Hitoshi Shiku, Shigenobu Kasai, Hiroyuki Noda, Tomokazu Matsue, Hiroaki Ohya-Nishiguchi, Hitoshi Kamada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Scanning electrochemical microscopy has been firstly used to map the enzymatic activity in natural plant tissues. The peroxidase (POD) was maintained in its original state in the celery (Apium graveolens L.) tissues and electrochemically visualized under its native environment. Ferrocenemethanol (FMA) was selected as a mediator to probe the POD in celery tissues based on the fact that POD catalyzed the oxidation of FMA by H2O2 to increase FMA+ concentration. Two-dimensional reduction current profiles for FMA+ produced images indicating the distribution and activity of the POD at the surface of the celery tissues. These images showed that the POD was widely distributed in the celery tissues, and larger amounts were found in some special regions such as the center of celery stem and around some vascular bundles.

Original languageEnglish
Pages (from-to)151-156
Number of pages6
JournalBioelectrochemistry
Volume54
Issue number2
DOIs
Publication statusPublished - 2001 Nov 3

Keywords

  • Celery tissues
  • Images
  • Peroxidase
  • Scanning electrochemical microscopy

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

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