Mapping of quantitative trait loci related to primary rice root growth as a response to inoculation with Azospirillum sp. strain B510

Sachiko Masuda, Kazuhiro Sasaki, Yuri Kazama, Chiharu Kisara, Shoko Takeda, Eiko Hanzawa, Kiwamu Minamisawa, Tadashi Sato

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1 Citation (Scopus)

Abstract

Azospirillum sp. strain B510 has been known as the plant growth-promoting endophyte; however, the growth-promotion effect is dependent on the plant genotype. Here, we aimed to identify quantitative trait loci (QTL) related to primary root length in rice at the seedling stage as a response to inoculation with B510. The primary root length of “Nipponbare” was significantly reduced by inoculation with B510, whereas that of “Kasalath” was not affected. Thus, we examined 98 backcrossed inbred lines and four chromosome segment substitution lines (CSSL) derived from a cross between Nipponbare and Kasalath. The primary root length was measured as a response to inoculation with B510, and the relative root length (RRL) was calculated based on the response to non-inoculation. Three QTL alleles, qRLI-6 and qRLC-6 on Chromosome (Chr.) 6 and qRRL-7 on Chr. 7 derived from Kasalath increased primary root length with inoculation (RLI), without inoculation, (RLC) and RRL and explained 20.2%, 21.3%, and 11.9% of the phenotypic variation, respectively. CSSL33, in which substitution occurred in the vicinity region of qRRL-7, showed a completely different response to inoculation with B510 compared with Nipponbare. Therefore, we suggest that qRRL-7 might strongly control root growth in response to inoculation with Azospirillum sp. strain B510.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalCommunicative and Integrative Biology
Volume11
Issue number3
DOIs
Publication statusPublished - 2018 May 4

Keywords

  • Azospirillum sp. strain B510
  • Primary rice root growth
  • plant genotype
  • plant growth-promoting bacteria
  • quantitative trait locus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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