Mapping of cholecystokinin transcription in transgenic mouse brain using Escherichia coil β-galactosidase reporter gene

Yasuhiro Itoh, Ikuo Kozakai, Masaaki Toyomizu, Teru Ishibashi, Ryozo Kuwano

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Cholecystokinin (CCK), a neuro-gut peptide, occurs not only in the nervous but also in the digestive system. As a first step in elucidating whether CCK gene expression and its physiological functions co-operate in these separate organs, transgenic mice were produced using CCK promoter that directs bacterial β-galactosidase as a reporter gene. A new transgenic vector was constructed, inserting the SV40 poly A signal 5' to the CCK promoter to impede any transcription upstream of the transgene. A 2.4 kb.p. region upstream to the transcription start site of the mouse CCK gene was used as the promoter. Transgene expression was detected first at embryonic 13.5 days in the central nervous system and increased after birth. The distribution of cells expressing β-galactosidase transgene agreed fairly well with that of in situ hybridization. In addition, the upregulation of CCK gene expression was clearly demonstrated by expressing β-galactosidase after injury to the brain. These results indicated that the 2.4 kb.p. of the CCK gene promoter region was sufficient to direct appropriate tissue-specific gene expression in mice.

Original languageEnglish
Pages (from-to)395-402
Number of pages8
JournalDevelopment Growth and Differentiation
Volume40
Issue number4
DOIs
Publication statusPublished - 1998

Keywords

  • Cholecystokinin
  • Mouse brain
  • Transcription
  • Transgenic mouse
  • β-galactosidase

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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