Mannose-binding lectin inhibits the motility of pathogenic salmonella by affecting the driving forces of motility and the chemotactic response

Jun Xu, Shuichi Nakamura, Md Shafiqul Islam, Yijie Guo, Kohei Ihara, Rintaro Tomioka, Mizuki Masuda, Hiroshi Yoneyama, Emiko Isogai

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Mannose-binding lectin (MBL) is a key pattern recognition molecule in the lectin pathway of the complement system, an important component of innate immunity. MBL functions as an opsonin which enhances the sequential immune process such as phagocytosis. We here report an inhibitory effect of MBL on the motility of pathogenic bacteria, which occurs by affecting the energy source required for motility and the signaling pathway of chemotaxis. When Salmonella cells were treated with a physiological concentration of MBL, their motile fraction and free-swimming speed decreased. Rotation assays of a single flagellum showed that the flagellar rotation rate was significantly reduced by the addition of MBL. Measurements of the intracellular pH and membrane potential revealed that MBL affected a driving force for the Salmonella flagellum, the electrochemical potential difference of protons. We also found that MBL treatment increased the reversal frequency of Salmonella flagellar rotation, which interfered with the relative positive chemotaxis toward an attractive substrate. We thus propose that the motility inhibition effect of MBL may be secondarily involved in the attack against pathogens, potentially facilitating the primary role of MBL in the complement system.

Original languageEnglish
Article numbere0154165
JournalPloS one
Volume11
Issue number4
DOIs
Publication statusPublished - 2016 Apr

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Mannose-binding lectin inhibits the motility of pathogenic salmonella by affecting the driving forces of motility and the chemotactic response'. Together they form a unique fingerprint.

Cite this