Mannose-binding lectin from yam (Dioscorea batatas) tubers with insecticidal properties against Helicoverpa armigera (Lepidoptera: Noctuidae)

Yuki Ohizumi, Mariam Gaidamashvili, Shyuichi Ohwada, Kazuhiro Matsuda, Junko Kominami, Sachiko Nakamura-Tsuruta, Jun Hirabayashi, Takako Naganuma, Tomohisa Ogawa, Koji Muramoto

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The amino acid sequence of mannose-binding lectin, named DB1, from the yam (Dioscorea batatas, synonym Dioscorea polystachya) tubers was determined. The lectin was composed of two isoforms DB1(Cys86) and DB1(Leu86) consisting of 108 amino acid residues with 90% sequence homology between them. DB1 showed a high sequence similarity to snowdrop (Galanthus nivalis) bulb lectin, GNA; especially, the carbohydrate-binding sites of GNA were highly conserved in DB1 DB1 interacted with D-mannose residues of oligosaccharides, and the oligosaccharides carrying two mannose-α-1,3-D-mannose units showed high binding affinity. DB1 was examined for insecticidal activity against Helicoverpa armigera (Lepidoptera: Noctuidae) larvae at different stages of development. The rate of adults successfully emerging from pupae fed on DB1 was 33%, when incorporated into an artificial diet at a level of 0.01% (w/w). Although DB1 had no or marginal inhibitory effects on gut proteolytic and glycolic enzymes, the lectin strongly bound to larval brush border and peritrophic membrane detected by immunostaining. The results show that DB1 may fulfill a defense role against insect pests.

Original languageEnglish
Pages (from-to)2896-2902
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number7
DOIs
Publication statusPublished - 2009 Apr 8

Keywords

  • Dioscorea batatas
  • Helicoverpa armigera
  • Insecticidal activity
  • Mannose-binding lectin
  • Yam

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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    Ohizumi, Y., Gaidamashvili, M., Ohwada, S., Matsuda, K., Kominami, J., Nakamura-Tsuruta, S., Hirabayashi, J., Naganuma, T., Ogawa, T., & Muramoto, K. (2009). Mannose-binding lectin from yam (Dioscorea batatas) tubers with insecticidal properties against Helicoverpa armigera (Lepidoptera: Noctuidae). Journal of Agricultural and Food Chemistry, 57(7), 2896-2902. https://doi.org/10.1021/jf8040269