Manipulation of microparticles for construction of array patterns by negative dielectrophoresis using multilayered array and grid electrodes

Kosuke Ino, Hitoshi Shiku, Fumisato Ozawa, Tomoyuki Yasukawa, Tomokazu Matsue

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, a useful method was developed to fabricate array patterns of microparticles not on electrode surfaces, but on arbitrary surfaces, using negative-dielectrophoresis (n-DEP). First, electrodes were designed and electric field simulations were performed to manipulate microparticles toward target areas. Based on the simulation results, multilayered array and grid (MLAG) electrodes, consisting of array electrodes surrounded by insulated regions and a grid electrode, were fabricated for the formation of localized, non-uniform electric fields. The MLAG electrode was mounted to a target substrate in a face-to-face configuration with a spacer. When an AC voltage (4.60 V rms and 1 MHz) was applied to the MLAG electrode, array patterns of 6 and 20 mm diameter microparticles were rapidly fabricated on the target substrate with ease. The results suggest that MLAG electrodes can be widely applied for the fabrication of biochips including cell arrays.

Original languageEnglish
Pages (from-to)709-718
Number of pages10
JournalBiotechnology and Bioengineering
Volume104
Issue number4
DOIs
Publication statusPublished - 2009 Nov 1

Keywords

  • Array patterns of microparticles
  • Manipulation of microparticles
  • Multilayered electrodes
  • Negative dielectrophoresis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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