Manipulating gene expressions by electroporation in the developing brain of mammalian embryos

Masanori Takahashi, Kenichi Sato, Tadashi Nomura, Noriko Osumi

Research output: Contribution to journalReview articlepeer-review

58 Citations (Scopus)

Abstract

One of the goals of developmental neuroscience in the post-genomic era is to clarify functions of a huge number of anonymous genes of which only DNA sequences are identified. More convenient methods for genetic manipulation in vertebrates, especially mammals, could help us to identify functions of the novel genes. Here we introduce a novel gene transfer technology using electroporation (EP), which is a simple and powerful strategy for genetic analysis. We have applied this method to cultured mammalian embryos in order to understand the function of specific genes in the developing brain. We have also performed EP in developing fetuses in utero guided by ultrasound image. The combination of these techniques in addition to analysis of genetic mutants will clarify functions of individual genes, gene interactions, and the molecular mechanisms underlying the brain development.

Original languageEnglish
Pages (from-to)155-162
Number of pages8
JournalDifferentiation
Volume70
Issue number4-5
DOIs
Publication statusPublished - 2002 Jun

Keywords

  • Electroporation
  • Mammalian brain
  • Ultrasound guided manipulation
  • Whole-embryo culture
  • in utero manipulation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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