Mallotus philippinensis bark extracts promote preferential migration of mesenchymal stem cells and improve wound healing in mice

Tadashi Furumoto, Noriyasu Ozawa, Yuta Inami, Misaki Toyoshima, Kosuke Fujita, Kaori Zaiki, Shunya Sahara, Mariko Akita, Keiko Kitamura, Koichi Nakaoji, Kazuhiko Hamada, Katsuto Tamai, Yasufumi Kaneda, Akito Maeda

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In the present study, we report the effects of the ethanol extract from Mallotus philippinensis bark (EMPB) on mesenchymal stem cell (MSC) proliferation, migration, and wound healing in vitro and in a mouse model. Chemotaxis assays demonstrated that EMPB acted an MSC chemoattractant and that the main chemotactic activity of EMPB may be due to the effects of cinnamtannin B-1. Flow cytometric analysis of peripheral blood mononuclear cells in EMPB-injected mice indicated that EMPB enhanced the mobilization of endogenous MSCs into blood circulation. Bioluminescent whole-animal imaging of luciferase-expressing MSCs revealed that EMPB augmented the homing of MSCs to wounds. In addition, the efficacy of EMPB on migration of MSCs was higher than that of other skin cell types, and EMPB treatment improved of wound healing in a diabetic mouse model. The histopathological characteristics demonstrated that the effects of EMPB treatment resembled MSC-induced tissue repair. Taken together, these results suggested that EMPB activated the mobilization and homing of MSCs to wounds and that enhancement of MSC migration may improve wound healing.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalPhytomedicine
Volume21
Issue number3
DOIs
Publication statusPublished - 2014 Feb 15
Externally publishedYes

Keywords

  • Mallotus philippinensis
  • Mesenchymal stem cells
  • Migration
  • Phenolic compounds
  • Wound healing

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

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