Maitotoxin-photoactive probe binds to membrane proteins in blood cells

Keiichi Konoki, Masaki Hashimoto, Kaori Honda, Kazuo Tachibana, Rie Tamate, Futoshi Hasegawa, Tohru Oishi, Michio Murata

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The photoactive and biotinylating ligand was prepared from MTX and maleimide-conjugated Hatanaka reagent with use of Diels-Alder reaction. Blood cells were subjected to affinity labelling experiments using the ligand thus obtained. The labelled band on SDS-PAGE was replaced not by MTX but by brevetoxin B (PbTx2), which suggested the presence of binding proteins in blood 2+ cells. Screening of polyether compounds for MTX inhibitory activity using Ca flux assays in C6 cells disclosed that a synthetic fragment of the hydrophilic portion of MTX inhibited the MTX activity.

Original languageEnglish
Pages (from-to)1007-1017
Number of pages11
JournalHeterocycles
Volume79
Issue numberC
DOIs
Publication statusPublished - 2009 Jul 29
Externally publishedYes

Keywords

  • Brevetoxin
  • Maitotoxin
  • Photoaffinity Labelling
  • Polyether Compound
  • Yessotoxin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology
  • Organic Chemistry

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