Maitotoxin-photoactive probe binds to membrane proteins in blood cells

Keiichi Konoki; Masaki Hashimoto; Kaori Honda; Kazuo Tachibana; Rie Tamate; Futoshi Hasegawa; Tohru Oishi; Michio Murata

doi:10.3987/COM-08-S(D)78

Maitotoxin-photoactive probe binds to membrane proteins in blood cells

Keiichi Konoki, Masaki Hashimoto, Kaori Honda, Kazuo Tachibana, Rie Tamate, Futoshi Hasegawa, Tohru Oishi, Michio Murata

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The photoactive and biotinylating ligand was prepared from MTX and maleimide-conjugated Hatanaka reagent with use of Diels-Alder reaction. Blood cells were subjected to affinity labelling experiments using the ligand thus obtained. The labelled band on SDS-PAGE was replaced not by MTX but by brevetoxin B (PbTx2), which suggested the presence of binding proteins in blood 2+ cells. Screening of polyether compounds for MTX inhibitory activity using Ca flux assays in C6 cells disclosed that a synthetic fragment of the hydrophilic portion of MTX inhibited the MTX activity.

Original language	English
Pages (from-to)	1007-1017
Number of pages	11
Journal	Heterocycles
Volume	79
Issue number	C
DOIs	https://doi.org/10.3987/COM-08-S(D)78
Publication status	Published - 2009 Jul 29
Externally published	Yes

Keywords

Brevetoxin
Maitotoxin
Photoaffinity Labelling
Polyether Compound
Yessotoxin

ASJC Scopus subject areas

Analytical Chemistry
Pharmacology
Organic Chemistry

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10.3987/COM-08-S(D)78

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title = "Maitotoxin-photoactive probe binds to membrane proteins in blood cells",

abstract = "The photoactive and biotinylating ligand was prepared from MTX and maleimide-conjugated Hatanaka reagent with use of Diels-Alder reaction. Blood cells were subjected to affinity labelling experiments using the ligand thus obtained. The labelled band on SDS-PAGE was replaced not by MTX but by brevetoxin B (PbTx2), which suggested the presence of binding proteins in blood 2+ cells. Screening of polyether compounds for MTX inhibitory activity using Ca flux assays in C6 cells disclosed that a synthetic fragment of the hydrophilic portion of MTX inhibited the MTX activity.",

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doi = "10.3987/COM-08-S(D)78",

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T1 - Maitotoxin-photoactive probe binds to membrane proteins in blood cells

AU - Konoki, Keiichi

AU - Hashimoto, Masaki

AU - Honda, Kaori

AU - Tachibana, Kazuo

AU - Tamate, Rie

AU - Hasegawa, Futoshi

AU - Oishi, Tohru

AU - Murata, Michio

PY - 2009/7/29

Y1 - 2009/7/29

N2 - The photoactive and biotinylating ligand was prepared from MTX and maleimide-conjugated Hatanaka reagent with use of Diels-Alder reaction. Blood cells were subjected to affinity labelling experiments using the ligand thus obtained. The labelled band on SDS-PAGE was replaced not by MTX but by brevetoxin B (PbTx2), which suggested the presence of binding proteins in blood 2+ cells. Screening of polyether compounds for MTX inhibitory activity using Ca flux assays in C6 cells disclosed that a synthetic fragment of the hydrophilic portion of MTX inhibited the MTX activity.

AB - The photoactive and biotinylating ligand was prepared from MTX and maleimide-conjugated Hatanaka reagent with use of Diels-Alder reaction. Blood cells were subjected to affinity labelling experiments using the ligand thus obtained. The labelled band on SDS-PAGE was replaced not by MTX but by brevetoxin B (PbTx2), which suggested the presence of binding proteins in blood 2+ cells. Screening of polyether compounds for MTX inhibitory activity using Ca flux assays in C6 cells disclosed that a synthetic fragment of the hydrophilic portion of MTX inhibited the MTX activity.

KW - Brevetoxin

KW - Maitotoxin

KW - Photoaffinity Labelling

KW - Polyether Compound

KW - Yessotoxin

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Maitotoxin-photoactive probe binds to membrane proteins in blood cells

Abstract

Keywords

ASJC Scopus subject areas

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