MafG sumoylation is required for active transcriptional repression

Hozumi Motohashi, Fumiki Katsuoka, Chika Miyoshi, Yasuhiro Uchimura, Hisato Saitoh, Claire Francastel, James Douglas Engel, Masayuki Yamamoto

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

A straightforward mechanism for eliciting transcriptional repression would be to simply block the DNA binding site for activators. Such passive repression is often mediated by transcription factors that lack an intrinsic repressor activity. MafG is a bidirectional regulator of transcription, a repressor in its homodimeric state but an activator when heterodimerized with p45. Here, we report that MafG is conjugated to SUMO-2/3 in vivo. To clarify the possible physiological role(s) for sumoylation in regulating MafG activity, we evaluated mutant and wild-type MafG in transgenic mice and cultured cells. Whereas sumoylation-deflcient MafG activated p45-dependent transcription normally and did not affect heterodimer activity, repression by the sumoylation-deficient MafG mutant was severely compromised in vivo. Furthermore, the SUMO-dependent repression activity of MafG was sensitive to histone deacetylase inhibition. Thus, repression by MafG is not achieved through simple passive repression by competing for the activator binding site but requires sumoylation, which then mediates transcriptional repression through recruitment of a repressor complex containing histone deacetylase activity.

Original languageEnglish
Pages (from-to)4652-4663
Number of pages12
JournalMolecular and cellular biology
Volume26
Issue number12
DOIs
Publication statusPublished - 2006 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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