MafA is a key regulator of glucose-stimulated insulin secretion

Chuan Zhang, Takashi Moriguchi, Miwako Kajihara, Ritsuko Esaki, Ayako Harada, Homare Shimohata, Hisashi Oishi, Michito Hamada, Naoki Morito, Kazuteru Hasegawa, Takashi Kudo, James Douglas Engel, Masayuki Yamamoto, Satoru Takahashi

Research output: Contribution to journalArticlepeer-review

324 Citations (Scopus)

Abstract

MafA is a transcription factor that binds to the promoter in the insulin gene and has been postulated to regulate insulin transcription in response to serum glucose levels, but there is no current in vivo evidence to support this hypothesis. To analyze the role of MafA in insulin transcription and glucose homeostasis in vivo, we generated MafA-deficient mice. Here we report that MafA mutant mice display intolerance to glucose and develop diabetes mellitus. Detailed analyses revealed that glucose-, arginine-, or KCl-stimulated insulin secretion from pancreatic β cells is severely impaired, although insulin content per se is not significantly affected. MafA-deficient mice also display age-dependent pancreatic islet abnormalities. Further analysis revealed that insulin 1, insulin 2, Pdx1, Beta2, and Glut-2 transcripts are diminished in MafA-deficient mice. These results show that MafA is a key regulator of glucose-stimulated insulin secretion in vivo.

Original languageEnglish
Pages (from-to)4969-4976
Number of pages8
JournalMolecular and cellular biology
Volume25
Issue number12
DOIs
Publication statusPublished - 2005 Jun

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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