Macrophages are the determinant of resistance to and outcome of nonlethal Babesia microti infection in mice

Mohamad Alaa Terkawi, Shinuo Cao, Maria S. Herbas, Maki Nishimura, Yan Li, Paul Franck Adjou Moumouni, Asadullah Hamid Pyarokhil, Daisuke Kondoh, Nobuo Kitamura, Yoshifumi Nishikawa, Kentaro Kato, Naoaki Yokoyama, Jinlin Zhou, Hiroshi Suzuki, Ikuo Igarashi, Xuenan Xuan

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16 Citations (Scopus)

Abstract

In the present study, we examined the contributions of macrophages to the outcome of infection with Babesia microti, the etiological agent of human and rodent babesiosis, in BALB/c mice. Mice were treated with clodronate liposome at different times during the course of B. microti infection in order to deplete the macrophages. Notably, a depletion of host macrophages at the early and acute phases of infection caused a significant elevation of parasitemia associated with remarkable mortality in the mice. The depletion of macrophages at the resolving and latent phases of infection resulted in an immediate and temporal exacerbation of parasitemia coupled with mortality in mice. Reconstituting clodronate liposome-treated mice at the acute phase of infection with macrophages from naive mice resulted in a slight reduction in parasitemia with improved survival compared to that of mice that received the drug alone. These results indicate that macrophages play a crucial role in the control of and resistance to B. microti infection in mice. Moreover, analyses of host immune responses revealed that macrophage-depleted mice diminished their production of Th1 cell cytokines, including gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α). Furthermore, depletion of macrophages at different times exaggerated the pathogenesis of the infection in deficient IFN-γ-/- and severe combined immunodeficiency (SCID) mice. Collectively, our data provide important clues about the role of macrophages in the resistance and control of B. microti and imply that the severity of the infection in immunocompromised patients might be due to impairment of macrophage function.

Original languageEnglish
Pages (from-to)8-16
Number of pages9
JournalInfection and immunity
Volume83
Issue number1
DOIs
Publication statusPublished - 2015

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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    Terkawi, M. A., Cao, S., Herbas, M. S., Nishimura, M., Li, Y., Adjou Moumouni, P. F., Pyarokhil, A. H., Kondoh, D., Kitamura, N., Nishikawa, Y., Kato, K., Yokoyama, N., Zhou, J., Suzuki, H., Igarashi, I., & Xuan, X. (2015). Macrophages are the determinant of resistance to and outcome of nonlethal Babesia microti infection in mice. Infection and immunity, 83(1), 8-16. https://doi.org/10.1128/IAI.02128-14