M-opsin protein degradation is inhibited by MG-132 in Rpe65 -/- retinal explant culture

Kota Sato, Taku Ozaki, Sei Ichi Ishiguro, Mitsuru Nakazawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Purpose: The 65 kDa retinal pigment epithelium-specific protein, RPE65, is an essential enzyme for 11-cis-retinal synthesis in the eye. Mutations of the RPE65 gene in humans result in severe vision loss, and Rpe65 -/- mice show early cone photoreceptor degeneration. We used an explant culture system to evaluate whether posttranslational downregulation of M-opsin protein in Rpe65 -/- mice is caused by proteolytic degradation. Methods: The eyes of three-week-old Rpe65 -/- mice were incubated in culture medium. Western blot analysis was used to evaluate the level of M-opsin protein, and immunofluorescence was used for protein localization. The transcriptional level of M-opsin was evaluated with real-time reverse-transcriptase-PCR. Results: Degradation of the M-opsin protein in Rpe65 -/- mouse retina was inhibited by the proteasome inhibitor MG-132 but not by the lysosomal inhibitor pepstatin A and E64d. 9-cis-retinal, used as an analog of 11-cis-retinal, increased Mopsin protein but did not increase M-opsin mRNA. Moreover, 9-cis-retinal did not change the transcriptional levels of photoreceptor specific genes. Conclusions: Our data suggest that M-opsin protein was degraded through a proteasome pathway and that M-opsin degradation was suppressed with 9-cis-retinal treatment in Rpe65 -/- mice to some extent.

Original languageEnglish
Pages (from-to)1516-1525
Number of pages10
JournalMolecular vision
Volume18
Publication statusPublished - 2012 Jun 13
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

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