Lysophosphatidylmethanol is a pan lysophosphatidic acid receptor agonist and is produced by autotaxin in blood

Tomoko Endo, Kuniyuki Kano, Rie Motoki, Kotaro Hama, Shinichi Okudaira, Mayuko Ishida, Hideo Ogiso, Masayuki Tanaka, Norio Matsuki, Ryo Taguchi, Motomu Kanai, Masakatsu Shibasaki, Hiroyuki Arai, Junken Aoki

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Lysophosphatidic acid (LPA) is a simple phospholipid but has numerous biological effects through a series of G-protein-coupled receptors specific to LPA. In general, LPA is short-lived when applied in vivo, which hinders most pharmacological experiments. In our continuing study to identify stable LPA analogues capable of in vivo applications, we identified here lysophosphatidylmethanol (LPM) as a stable and pan-LPA receptor agonist. A synthetic LPM activated all five LPA receptors (LPA1-5), and stimulates both cell proliferation and LPA-receptor-dependent cell motility. In addition, LPM showed a hypertensive effect in rodent when applied in vivo. We found that, when fetal calf serum was incubated in the presence of methanol, formation of LPM occurred rapidly, whereas it was completely blocked by depletion of autotaxin (ATX), a plasma enzyme that converts lysophosphatidylcholine (LPC) to LPA. When recombinant ATX was incubated with LPC in the presence of methanol, both LPM and LPA were produced with a ratio of 1:10, showing that ATX has transphosphatidylation activity in addition to its lysophospholipase D activity. Administration of methanol in mice resulted in the formation of several micromoles of LPM in plasma, which is much higher than that of LPA. The present study identified LPM as a novel and stable lysophospholipid mediator with LPA-like activities and ATX as a potential synthetic enzyme for LPM.

Original languageEnglish
Pages (from-to)283-293
Number of pages11
JournalJournal of biochemistry
Volume146
Issue number2
DOIs
Publication statusPublished - 2009 Aug

Keywords

  • Autotaxin
  • Lysophosphatidic acid
  • Lysophosphatidylcholine
  • Lysophosphatidylmethanol
  • Transphosphatidylation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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