TY - JOUR
T1 - Lymphatic mapping of mice with systemic lymphoproliferative disorder
T2 - Usefulness as an inter-lymph node metastasis model of cancer
AU - Shao, Lenan
AU - Mori, Shiro
AU - Yagishita, Yoko
AU - Okuno, Tatsuki
AU - Hatakeyama, Yuriko
AU - Sato, Takuma
AU - Kodama, Tetsuya
N1 - Funding Information:
M. Shiro acknowledges a Grant-in-Aid for Scientific Research (B) (22390378) and a Grant-in-Aid for Challenging Exploratory Research (24659884). T. Kodama acknowledges a Grant-in-Aid for Scientific Research (B) (23300183) and a Grant-in-Aid for Challenging Exploratory Research (24650286). Special thanks are expressed to Masao Nose for pathological discussions and for assistance in preparing the manuscript.
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/3/29
Y1 - 2013/3/29
N2 - Preclinical models of lymph node (LN) metastasis are fundamental to the study and design of new techniques for the diagnosis and treatment of LN metastasis. However, the identification of LNs and lymphatic vessels (LVs) in mice is challenging with conventional imaging modalities, since the LN diameter in normal mice is 1-2. mm. Here, we describe MXH10/Mo lpr/. lpr (MXH10/Mo/lpr) inbred mice, which develop systemic swelling of LNs up to 10. mm in diameter, allowing investigation of the topography of LNs and LVs. Using a gross anatomy dissection approach, we identified 22 different LNs situated in the head and neck, limbs, thoracic and abdominal regions. Furthermore, four peripheral inter-LN vessels were found: from the subiliac LN (SiLN) to the proper axillary LN (PALN); from the parotid LN to the caudal deep cervical LN; and from the popliteal LN to both the sciatic LN and the SiLN. Metastasis to the PALN via LVs was induced by inoculating FM3A/. Luc mouse mammary carcinoma cells into the SiLN. Our results demonstrate that the MXH10/Mo/lpr mouse strain is an excellent model in which to investigate lymphatic drainage and inter-LN metastasis of cancer. This paper unveils the anatomy of murine lymphatics to give new insights into the investigation of inter-LN metastasis of cancer, especially the mechanisms involved in the trafficking of cancer cells through inter-LN vessels. The results provide data that may prove very useful in the quest to develop better lymph drainage-based drug delivery systems.
AB - Preclinical models of lymph node (LN) metastasis are fundamental to the study and design of new techniques for the diagnosis and treatment of LN metastasis. However, the identification of LNs and lymphatic vessels (LVs) in mice is challenging with conventional imaging modalities, since the LN diameter in normal mice is 1-2. mm. Here, we describe MXH10/Mo lpr/. lpr (MXH10/Mo/lpr) inbred mice, which develop systemic swelling of LNs up to 10. mm in diameter, allowing investigation of the topography of LNs and LVs. Using a gross anatomy dissection approach, we identified 22 different LNs situated in the head and neck, limbs, thoracic and abdominal regions. Furthermore, four peripheral inter-LN vessels were found: from the subiliac LN (SiLN) to the proper axillary LN (PALN); from the parotid LN to the caudal deep cervical LN; and from the popliteal LN to both the sciatic LN and the SiLN. Metastasis to the PALN via LVs was induced by inoculating FM3A/. Luc mouse mammary carcinoma cells into the SiLN. Our results demonstrate that the MXH10/Mo/lpr mouse strain is an excellent model in which to investigate lymphatic drainage and inter-LN metastasis of cancer. This paper unveils the anatomy of murine lymphatics to give new insights into the investigation of inter-LN metastasis of cancer, especially the mechanisms involved in the trafficking of cancer cells through inter-LN vessels. The results provide data that may prove very useful in the quest to develop better lymph drainage-based drug delivery systems.
KW - Cancer metastasis
KW - Inter-lymph node vessels
KW - Lymph drainage-based drug delivery
KW - Lymphatic mapping
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U2 - 10.1016/j.jim.2013.01.004
DO - 10.1016/j.jim.2013.01.004
M3 - Article
C2 - 23328410
AN - SCOPUS:84873714572
VL - 389
SP - 69
EP - 78
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1-2
ER -