Lymphatic mapping of mice with systemic lymphoproliferative disorder: Usefulness as an inter-lymph node metastasis model of cancer

Lenan Shao, Shiro Mori, Yoko Yagishita, Tatsuki Okuno, Yuriko Hatakeyama, Takuma Sato, Tetsuya Kodama

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)


Preclinical models of lymph node (LN) metastasis are fundamental to the study and design of new techniques for the diagnosis and treatment of LN metastasis. However, the identification of LNs and lymphatic vessels (LVs) in mice is challenging with conventional imaging modalities, since the LN diameter in normal mice is 1-2. mm. Here, we describe MXH10/Mo lpr/. lpr (MXH10/Mo/lpr) inbred mice, which develop systemic swelling of LNs up to 10. mm in diameter, allowing investigation of the topography of LNs and LVs. Using a gross anatomy dissection approach, we identified 22 different LNs situated in the head and neck, limbs, thoracic and abdominal regions. Furthermore, four peripheral inter-LN vessels were found: from the subiliac LN (SiLN) to the proper axillary LN (PALN); from the parotid LN to the caudal deep cervical LN; and from the popliteal LN to both the sciatic LN and the SiLN. Metastasis to the PALN via LVs was induced by inoculating FM3A/. Luc mouse mammary carcinoma cells into the SiLN. Our results demonstrate that the MXH10/Mo/lpr mouse strain is an excellent model in which to investigate lymphatic drainage and inter-LN metastasis of cancer. This paper unveils the anatomy of murine lymphatics to give new insights into the investigation of inter-LN metastasis of cancer, especially the mechanisms involved in the trafficking of cancer cells through inter-LN vessels. The results provide data that may prove very useful in the quest to develop better lymph drainage-based drug delivery systems.

Original languageEnglish
Pages (from-to)69-78
Number of pages10
JournalJournal of Immunological Methods
Issue number1-2
Publication statusPublished - 2013 Mar 29


  • Cancer metastasis
  • Inter-lymph node vessels
  • Lymph drainage-based drug delivery
  • Lymphatic mapping

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Lymphatic mapping of mice with systemic lymphoproliferative disorder: Usefulness as an inter-lymph node metastasis model of cancer'. Together they form a unique fingerprint.

Cite this