Luminol chemiluminescent determination of hydrogen peroxide at picomole levels using high-performance liquid chromatography with a cation-exchange resin gel column

Teruo Miyazawa, Sittiwat Lertsiri, Kenshiro Fujimoto, Michiko Oka

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The optimum conditions of high-performance liquid chromatography combined with luminol chemiluminescence detection were established for the determination of hydrogen peroxide at picomole levels using a cation-exchange gel column. The gel column with distilled water as the mobile phase allowed a good separation of H2O2 without causing any irreversible binding of H2O2 to the column surface. The detection limit and the quantification limit of H2O2 were 4 and 6-600 pmol, respectively. The suitability of the present method was verified by the determination of H2O2 present in coffee drinks.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalJournal of Chromatography A
Volume667
Issue number1-2
DOIs
Publication statusPublished - 1994 Apr 29

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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