Lsi-based amperometric device for electrochemical imaging of drug effect on dopamine release from three-dimensional cultured pc12 cells

H. Abe, K. Ino, C. Z. Li, Y. Kanno, K. Y. Inoue, A. Suda, R. Kunikata, M. Matsudaira, Y. Takahashi, H. Shiku, T. Matsue

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a large-scale integration (LSI) based amperometric chip device containing 400 sensors with a pitch of 250 μm and its applications for biological analysis of dopamine release from three-dimensional (3D) cultured cells using rat pheochromocytoma (PC12) cells as a neural cell model. The 3D cultured PC12 cells (PC12 spheroids) were stimulated with a high K+ solution to induce dopamine release. We showed clearly drug effects of dopaminergic drugs L-3,4-dihydroxyphenylalanine, (L-DOPA) and reserpine on dopamine release from PC12 spheroids. Our results indicate that the device is a useful tool for evaluating the effects of dopaminergic drugs.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1598-1600
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015 Jan 1
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • CMOS
  • Neurotransmitter
  • Spheroid

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Abe, H., Ino, K., Li, C. Z., Kanno, Y., Inoue, K. Y., Suda, A., Kunikata, R., Matsudaira, M., Takahashi, Y., Shiku, H., & Matsue, T. (2015). Lsi-based amperometric device for electrochemical imaging of drug effect on dopamine release from three-dimensional cultured pc12 cells. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1598-1600). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.