Lowered glucose suppressed the proliferation and increased the differentiation of murine neural stem cells in vitro

Nobutaka Horie, Takahiro Moriya, Masato Mitome, Naoki Kitagawa, Izumi Nagata, Kazuyuki Shinohara

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Cerebral ischemia is known to activate endogenous neural stem cells (NSCs), but its mechanisms remain unknown. Since lowered glucose supply seems to mediate ischemic actions, we examined the effect of low glucose on NSC activities in vitro. Low glucose applied during the proliferation period diminished EGF-induced proliferation of NSCs without affecting subsequent differentiation, but low glucose directly exposed during the differentiation period facilitated the differentiation of NSCs into neurons and astrocytes. These findings suggest that low glucose facilitated NSC differentiation, but it diminished NSC proliferation. Moreover, the effect of low glucose may be dependent on the timing of application.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalFEBS Letters
Volume571
Issue number1-3
DOIs
Publication statusPublished - 2004 Jul 30

Keywords

  • BrdU, bromodeoxyuridine
  • DMEM, Dulbecco's modified Eagle's medium
  • Differentiation
  • Hypoglycemia
  • Ischemia
  • NSCs, neural stem cells
  • Neural stem cell
  • Proliferation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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