Low molecular weight immunosuppressive factors found in elevated amounts in cancer ascitic fluids of mice 1. Isolation, identification and immunosuppressive effects of uric acid and uracil

Shunsuke Saim, Shoichi Takano, Toshiro Majima, Hisashi Aso, Takehiko Nakamura, Nakao Ishida

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

A definite increase in two low molecular weight factors, G10-2 and G10-3 was found in Ehrlich ascitic fluids, parallel to tumor growth. The isolation and identification of the two factors were attempted through gel filtration and reversed phase column chromatography, using ascitic fluids obtained 13 days after intraperitoneal implantation of Ehrlich tumor cells. As a result, two highly purified factors were observed upon examination by high performance liquid chromatography. Additional analytical data, collected by UV spectrum, NMR spectrum and mass analysis. allowed us to identify G10-2 as uric acid and G10-3 as uracil. Detailed immunological analysis of uric acid and uracil revealed that the augmenting activities of mouse and human NK cells by mouse IFNδbeta; or human rIFN δA/D were impaired in the presence of either compound at concentrations of 0.07 mM, the concentration detectable in the ascitic fluid of tumor bearing mice.

Original languageEnglish
Pages (from-to)39-58
Number of pages20
JournalImmunopharmacology and Immunotoxicology
Volume8
Issue number1
DOIs
Publication statusPublished - 1986 Jan 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Toxicology
  • Pharmacology

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