Low-level chemiluminescence and life span of Drosophila melanogaster

Tomoko Sato, Teruo Miyazawa, Masaki Kobayashi, Hideyuki Furukawa, Humio Inaba

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Spontaneous photon emission (chemiluminescence, CL) as a monitor of free radical evolution in Drosophila melanogaster which had been maintained at 25 or 30°C for 5 days after emergence was measured. When maintained at 30°C the fly CL intensity was stronger than at 25°C. Under the condition of the higher temperature, the fly life span was shorter (mean life span = 29 days at 30°C and 63 days at 25°C), and oxygen consumption (3.7 μl/mg·h at 25°C, 4.9 μl/mg·h at 30°C) and the mobility (movement distance = 25 mm/min at 25°C, 700 mm/min at 30°C) increased, together with augmentation of phospholipid hydroperoxide in the fly total lipids. The CL spontaneously emitted from fly homogenate was decreased by the free radical scavengers both in experimtens in vivo and in vitro. The hypothesis is proposed that as the oxygen metabolism grows active, the chemiluminescent reactions that involve oxygen-dependent free radical metabolism, including membrane phospholipid hydroperoxidation, contribute to the acceleration of senescence of fly bodies.

Original languageEnglish
Pages (from-to)50-58
Number of pages9
JournalGerontology
Volume38
Issue number1-2
DOIs
Publication statusPublished - 1992 Jan 1

Keywords

  • Aging
  • Chemiluminescence
  • Drosophila
  • Free radical
  • Life span
  • Lipid peroxide

ASJC Scopus subject areas

  • Ageing
  • Geriatrics and Gerontology

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