TY - JOUR
T1 - Low infection of Phelipanche aegyptiaca in micro-tom mutants deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8
AU - Hasegawa, Shoko
AU - Tsutsumi, Takuya
AU - Fukushima, Shunsuke
AU - Okabe, Yoshihiro
AU - Saito, Junna
AU - Katayama, Mina
AU - Shindo, Masato
AU - Yamada, Yusuke
AU - Shimomura, Koichiro
AU - Yoneyama, Kaori
AU - Akiyama, Kohki
AU - Aoki, Koh
AU - Ariizumi, Tohru
AU - Ezura, Hiroshi
AU - Yamaguchi, Shinjiro
AU - Umehara, Mikihisa
N1 - Funding Information:
Funding: This work was in part supported by grants from Toyo University (the Inoue Enryo Memorial Foundation for Promoting Sciences to S.H.), and from the Japan Society for the Promotion of Science (KAKENHI Grant Nos. 17K07650 to M.U., 16K18560 to K.Y., and 16H04875 to K.A.), the Japan Science and Technology Agency PRESTO, and by a Cooperative Research Grant of the Plant Transgenic Design Initiative (PTradD) by the Gene Research Center, Tsukuba Plant Innovation Research Center, University of Tsukuba (to S.Y. and M.U.).
Funding Information:
Acknowledgments: We thank Yu Kasuo, Jiei Tanikawa, Atsuya Kubota, Karen Endo, and Nao Tsunoda (Toyo University) for their technical assistance, Koichi Yoneyama (Utsunomiya University) for providing O. minor seeds, Joseph Hershenhorn and Dina Plakhine (Newe-Ya’ar Research Center, Agriculture Research Organization, Israel) for providing P. aegyptiaca seeds, and Shosaku Kashiwada, Hiroki Higashibata, Yuichi Iwasaki, and Uma Maheswari Rajagopalan (Toyo University) for constructive comments. Tomato seeds (TOMJPF00001, TOMJPE7343, 7024, 8245, 7311, 5639, 7720, 8940, 2481, 5550, 3979, 5291, and 2757) were provided by the University of Tsukuba, Gene Research Center, through National Bio-Resource Project of the Japan Agency for Medical Research and Development. This work was in part supported by the Research Center for Life and Environmental Sciences, Toyo University.
Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2018/9/6
Y1 - 2018/9/6
N2 - Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa—hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the “targeting induced local lesions in genomes” (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca.
AB - Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa—hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the “targeting induced local lesions in genomes” (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca.
KW - Carotenoid cleavage dioxygenase 8
KW - Orobanche minor
KW - Phelipanche aegyptiaca
KW - Shoot branching
KW - Solanum lycopersicum
KW - Strigolactones
UR - http://www.scopus.com/inward/record.url?scp=85053078563&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85053078563&partnerID=8YFLogxK
U2 - 10.3390/ijms19092645
DO - 10.3390/ijms19092645
M3 - Article
C2 - 30200620
AN - SCOPUS:85053078563
SN - 1422-0067
VL - 19
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 9
M1 - 2645
ER -