Low assimilation efficiency of photorespiratory ammonia in conifer leaves

Shin Ichi Miyazawa, Mitsuru Nishiguchi, Norihiro Futamura, Tomohisa Yukawa, Mitsue Miyao, Tsuyoshi Emilio Maruyama, Takayuki Kawahara

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Glutamine synthetase (GS) localized in the chloroplasts, GS2, is a key enzyme in the assimilation of ammonia (NH3) produced from the photorespiration pathway in angiosperms, but it is absent from some coniferous species belonging to Pinaceae such as Pinus. We examined whether the absence of GS2 is common in conifers (Pinidae) and also addressed the question of whether assimilation efficiency of photorespiratory NH3 differs between conifers that may potentially lack GS2 and angiosperms. Search of the expressed sequence tag database of Cryptomeria japonica, a conifer in Cupressaceae, and immunoblotting analyses of leaf GS proteins of 13 species from all family members in Pinidae revealed that all tested conifers exhibited only GS1 isoforms. We compared leaf NH3 compensation point (γNH3) and the increments in leaf ammonium content per unit photorespiratory activity (NH3 leakiness), i.e. inverse measures of the assimilation efficiency, between conifers (C. japonica and Pinus densiflora) and angiosperms (Phaseolus vulgaris and two Populus species). Both γNH3 and NH3 leakiness were higher in the two conifers than in the three angiosperms tested. Thus, we concluded that the absence of GS2 is common in conifers, and assimilation efficiency of photorespiratory NH3 is intrinsically lower in conifer leaves than in angiosperm leaves. These results imply that acquisition of GS2 in land plants is an adaptive mechanism for efficient NH3 assimilation under photorespiratory environments.

Original languageEnglish
Pages (from-to)789-802
Number of pages14
JournalJournal of Plant Research
Volume131
Issue number5
DOIs
Publication statusPublished - 2018 Sep 1

Keywords

  • Ammonia
  • Angiosperm
  • Chloroplastic glutamine synthetase (GS2)
  • Conifer
  • NH compensation point
  • Photorespiration

ASJC Scopus subject areas

  • Plant Science

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