Lotus japonicus SUNERGOS1 encodes a predicted subunit A of a DNA topoisomerase VI that is required for nodule differentiation and accommodation of rhizobial infection

Hwi Joong Yoon, Md Shakhawat Hossain, Mark Held, Hongwei Hou, Marilyn Kehl, Alexandre Tromas, Shusei Sato, Satoshi Tabata, Stig Uggerhøj Andersen, Jens Stougaard, Loretta Ross, Krzysztof Szczyglowski

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17 Citations (Scopus)

Abstract

Summary A symbiotic mutant of Lotus japonicus, called sunergos1-1 (suner1-1), originated from a har1-1 suppressor screen. suner1-1 supports epidermal infection by Mesorhizobium loti and initiates cell divisions for organogenesis of nodule primordia. However, these processes appear to be temporarily stalled early during symbiotic interaction, leading to a low nodule number phenotype. This defect is ephemeral and near wild-type nodule numbers are reached by suner1-1 at a later point after infection. Using an approach that combined map-based cloning and next-generation sequencing we have identified the causative mutation and show that the suner1-1 phenotype is determined by a weak recessive allele, with the corresponding wild-type SUNER1 locus encoding a predicted subunit A of a DNA topoisomerase VI. Our data suggest that at least one function of SUNER1 during symbiosis is to participate in endoreduplication, which is an essential step during normal differentiation of functional, nitrogen-fixing nodules.

Original languageEnglish
Pages (from-to)811-821
Number of pages11
JournalPlant Journal
Volume78
Issue number5
DOIs
Publication statusPublished - 2014 Jun
Externally publishedYes

Keywords

  • Lotus japonicus
  • endocycle
  • legumes
  • symbiosis
  • topoisomerase

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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    Yoon, H. J., Hossain, M. S., Held, M., Hou, H., Kehl, M., Tromas, A., Sato, S., Tabata, S., Andersen, S. U., Stougaard, J., Ross, L., & Szczyglowski, K. (2014). Lotus japonicus SUNERGOS1 encodes a predicted subunit A of a DNA topoisomerase VI that is required for nodule differentiation and accommodation of rhizobial infection. Plant Journal, 78(5), 811-821. https://doi.org/10.1111/tpj.12520